We narrowed to 3,277 results for: pET28
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Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-SBiT_Strep-Tag-II
Plasmid#198750PurposeExpresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - SmallBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
Tags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389Available SinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp10_SARS-CoV-2
Plasmid#167851PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a SnoopTag-mEGFP-SpyTag
Plasmid#72325PurposeBacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.DepositorInsertpET28a SnoopTag-mEGFP-SpyTag
TagsN-terminal His6 tagExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-ULP1-405-621
Plasmid#229224PurposeFor expression of ULP1 proteinDepositorAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
His6-SUMO-pET28a-EfaCas1
Plasmid#112793PurposeTo express enterococcus faecalis Cas1 protein in E. coliDepositorInsertEfaCas1
TagsHis6-SUMOExpressionBacterialAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pet28a-His-tag-BS2
Plasmid#220347PurposeE. coli expression vector to purify BS2DepositorInsertBS2
TagsHis tagExpressionBacterialAvailable SinceJune 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
TagsHis6 and StrepExpressionBacterialPromoterT7Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogTag-MBP
Plasmid#171773PurposeExpresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogTag-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
TagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1 (273-427)
Plasmid#162734PurposeBacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1 domain residues 273-427
ExpressionBacterialMutationCodon optimisedAvailable SinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-Nsp14-TEV-Strep
Plasmid#154953PurposeExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coliDepositorAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
TagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialPromoterT7Available SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
TagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-6His-SUMO-Nsp10
Plasmid#154959PurposeExpresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coliDepositorInsert6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
Tags6His-Thrombin-SUMOExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28_mouse-Caspase-1_enzymatic-domain
Plasmid#183385PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only