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pEF myc-SHP2(Y279C)

Plasmid

#245230

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Y279C

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(E139D)

Plasmid

#245229

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

E139D

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510H)

Plasmid

#245234

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510H

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q506P)

Plasmid

#245232

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q506P

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF myc-SHP2(Q510E)

Plasmid

#245233

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

Q510E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(WT)

Plasmid

#245216

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

wild-type

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(Q510E)

Plasmid

#245218

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

Q510E

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(Q510E)

Plasmid

#245214

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

Q510E

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28b His6-TEV-SHP2-FL(Q510K)

Plasmid

#245215

Purpose

Production of His-tagged full-length SHP2

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 full-length

Tags

6xHis

Expression

Bacterial

Mutation

Q510K

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NIC-His6-TEV-SHP2-PTP(Q510K)

Plasmid

#245219

Purpose

Production of His-tagged SHP2 PTP domain

Depositor

Neel Shah

Article

van Vlimmeren et al bioRxiv [Preprint]. 2025 Jul 2:2025.06.3

Insert

SHP2 PTP domain

Tags

6xHis

Expression

Bacterial

Mutation

Q510K

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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HSA_3b*_AP

Plasmid

#238899

Purpose

Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

Recombinant Human Serum Albumin (HSA) 3b*

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_TEV_aMFx1 (4a)

Plasmid

#238903

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_TEV_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PiggyBac-APEX-V5-NES

Plasmid

#229082

Purpose

Doxycycline-inducible expression of nuclear export signal (NES) fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone

Depositor

Eran Hornstein

Insert

NES (TARDBP Human)

Tags

APEX (D11G mutation), BFP, and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF9-Pct3-(–)-Eldh2(cHis6)-Accs(ElA2)

Plasmid

#233868

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh2

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Tags

His

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)

Plasmid

#233876

Purpose

Expresses Eggerthella lenta A2 Dadh with the accessory genes from the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015910

Plasmid

#226287

Purpose

Expresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Isoeugenol synthase 1

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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