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Addgene
Showing: 821 - 840 of 1777 results
  1. MK1283

    Plasmid
    #71428
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709
    Depositor
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …
    Promoter
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. MK1274

    Plasmid
    #71431
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.
    Depositor
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…
    Promoter
    Available since
    Dec. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1mut
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pCP60 RS-GFP

    Plasmid
    #122173
    Purpose
    "Binary vector for Agrobacterium tumefacience-mediated transformation of plant cells resulting in constitutive expression of cytoplasmatically localized RS-GFP"
    Depositor
    Insert
    RS-GFP
    Use
    Binary vector for escherichia coli and agrobacter…
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    March 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pMSCV-IRES-mAmetrine 1.1 FP

    Plasmid
    #52113
    Purpose
    a bicistronic IRES-mAtetrine containing retroviral vector
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    IRES-mAmetrine 1.1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pUS232

    Plasmid
    #84579
    Purpose
    Shuttle and expression vector. Predicted to replicate in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Tetracycline-inducible expression of cloned genes.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 21, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. pRAN473

    Plasmid
    #120813
    Purpose
    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein
    Insert
    red fluorescent protein (mCherry), codon-optimized for C. difficile, multiple-cloning site for protein fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Ptet
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pHR_SFFV_tTA_IRES_tagBFP

    Plasmid
    #231953
    Purpose
    Lentiviral vector for constitutive expression of the tetracycline transactivator tTA and of the blue fluorescent protein tagBFP, separated by an internal ribosome entry site (SFFV promoter)
    Depositor
    Insert
    tTA_IRES_tagBFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. IBMc326

    Plasmid
    #161949
    Purpose
    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20
    Depositor
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques
    Depositor
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 821 - 840 of 1777 results