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pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85930

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-185-5p Sensor (MIR185 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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IDP427

Plasmid

#87021

Purpose

genomic Sindbis virus plasmid for use in SYNseq PLA screen

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

SNAP-Nlg1AB-tdMS2(i)
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHA-ΔN332-Kin1C

Plasmid

#31608

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

ΔN332 kinesin-1C (Kif5c Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
mc2 blast EF1a

Plasmid

#206223

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G08

Plasmid

#67142

Purpose

Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pORE303N_CEN12RRFuzzy3MYB

Plasmid

#194441

Purpose

CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

Depositor

Chung-Jui Tsai

Article

Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

Insert

gRNA array targeting CEN1, RR, Fuzzy3MYB

Use

CRISPR

Expression

Plant

Promoter

MtU6

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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mEos2-Calnexin-N-14

Plasmid

#57352

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calnexin (CANX Human)

Tags

mEos2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBS/KS-mId2sp

Plasmid

#16044

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id2 (Id2 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 1-195

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pFPL-Yh2

Plasmid

#61650

Purpose

Gateway vector for generating yellow fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced yellow fluorescent protein

Expression

Plant

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only
pGEMHE-mClover3

Plasmid

#171497

Purpose

T7 promotor drives in vitro transcription of mClover3 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

mClover3

Use

Pgehme

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH1

Plasmid

#168977

Purpose

AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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TAL2274

Plasmid

#36711

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Left (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
mNG-mTq2

Plasmid

#124217

Purpose

Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

mNG-mTq2

Expression

Bacterial

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_SIAH2_Sina

Plasmid

#109813

Purpose

Protein expression and purification of SIAH2_Sina

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SIAH2_Sina (SIAH2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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