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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,240 results for: Comp;

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Showing: 8421 - 8440 of 16240 results

  1. pcDNA3-ABKAR-Kras

    Plasmid
    #84637
    Purpose
    Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
    Tags
    6xHis, C-terminal targeting sequence from Kras, C…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    84637-plasmid-map-sequence-id-176126

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  4. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Article
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Insert
    ERKsub(TA)DD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available Since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

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  7. deltaVP1 + VP2C-GFP

    Plasmid
    #166676
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-GFP
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166676-plasmid-map-sequence-id-329830

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  11. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available Since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

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  16. pET28 His-MBP-TEV-BAP-Human BCKDK

    Plasmid
    #232116
    Purpose
    For bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    BCKDK (BCKDK Human)
    Tags
    6 x His, Biotin acceptor peptide (BAP), Maltose-B…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232116-plasmid-map-sequence-id-464924

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  17. pcDNA3.1-myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA

    Plasmid
    #228530
    Purpose
    Firefly luciferase in fusion with a fragment of human Skp1 protein through a flexible linker and a TEV viral protease cleavage site. Expression of fLuc protein is deinhibited by TEV site cleavage.
    Depositor
    Roman Jerala
    Article
    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80
    Insert
    myc:Skp1(dC111):gs6:TEVs:gs6:fLuc:HA (SKP1 Human, Photinus pyralis, Synthetic)
    Tags
    HA and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228530-plasmid-map-sequence-id-456690

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Showing: 8421 - 8440 of 16240 results