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pB-2x-lns-DEST (JDW 1206)

Plasmid

#229818

Purpose

A PiggyBac destination vector compatible with gateway with 2 core cHS4 insulators by each ITR site

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-WEE1shRNA1

Plasmid

#220571

Purpose

To inducibly knockdown WEE1 expression

Depositor

Sean Lee

Insert

WEE1 shRNA1 (WEE1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-WEE1shRNA2

Plasmid

#220572

Purpose

To inducibly knockdown WEE1 expression

Depositor

Sean Lee

Insert

WEE1 shRNA2 (WEE1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX-he1.1:YFP

Plasmid

#184816

Purpose

Used to generate the Tg(QUAS:GFP-CAAX; he1.1:YFP)c631 transgenic line

Depositor

Marnie Halpern

Publication

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:NLS-mApple-he1.1:CFP

Plasmid

#184814

Purpose

Used to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic line

Depositor

Marnie Halpern

Publication

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:NLS-mApple-pA; he1.1:CFP

Use

Zebrafish tol2 transgenesis

Tags

NLS-mApple

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BRIPO

Plasmid

#215542

Purpose

BRIPO (Bioluminescent Red Indicator for Potassium)

Depositor

Huiwang Ai

Publication

Zhao et al J Am Chem Soc. 2024 May 15;146(19):13406

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BRIPO

Expression

Mammalian

Promoter

CMV promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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EC67904

Plasmid

#209470

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas12a HH-DR-HDV

Use

CRISPR

Promoter

HvU3

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC67895

Plasmid

#209506

Purpose

Cloning protospacers for Cas12a editing

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas12a-tRNA-DR-DR

Use

CRISPR

Promoter

TaU6

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMS84 (U6p::GGACAGTCCTGCCGAGGTGG)

Plasmid

#193852

Purpose

Encodes the guide RNA targeting the sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Publication

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

Guide RNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P-BT1763

Plasmid

#188111

Purpose

pBolux harboring the promoter region upstream of BT1763

Depositor

Guy Townsend

Publication

Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

Insert

The B. thetaiotaomicron BT1763 promoter cloned into pBolux

Expression

Bacterial

Promoter

BT1763

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pc-GAKIN-Ha

Plasmid

#188246

Purpose

Express GAKIN in mammalian cells with Ha tag

Depositor

Joel Pomerantz

Publication

Lamason et al Mol Cell. 2010 Dec 10;40(5):798-809. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAKIN (KIF13B Human)

Tags

Ha

Expression

Mammalian

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA

Plasmid

#164795

Purpose

pLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter.

Depositor

Jonathan Ploski

Publication

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

4xMyc

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHWBF06exp

Plasmid

#130831

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Publication

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Tags

mCherry-FLAG-HA

Expression

Plant

Promoter

CaMV 35S

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDS1902

Plasmid

#167278

Purpose

Expression plasmid for Candida albicans containing a red-shifted firefly luciferase

Depositor

Dominique Sanglard

Publication

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

red shifted luciferase

Promoter

ACT1

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone32 (Opti-SpCas9)

Plasmid

#131736

Purpose

Expresses Opti-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Publication

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

Opti-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

R661A+K1003H

Promoter

EFS

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGB411

Plasmid

#134760

Purpose

pCambia CRISPR/Cas9

Depositor

Qi-Jun Chen

Publication

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPR/Cas9

Use

CRISPR

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pG3H-U6EC1

Plasmid

#134758

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Publication

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA-U6, zCas9-EC1

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_SPIC_P2A_Hygro_Barcode

Plasmid

#120484

Purpose

Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SPIC (SPIC Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-HMGS

Plasmid

#108945

Purpose

heterologous expression of HMGS of E. faecalis in E. coli

Depositor

Frank Schulz

Publication

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA synthase (HMGS)

Tags

His6-Tag

Expression

Bacterial

Mutation

A110G

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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LI LacZ

Plasmid

#104528

Purpose

LI lacZ module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

lacZ

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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