We narrowed to 32,570 results for: Eng

1. pNJB99

   Plasmid

   #51521

   Purpose

   Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Insert

   Zif268:FokI
   

   Use

   Gateway entry vector

   Tags

   NLS

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS-PesaR-Dlux

   Plasmid

   #47653

   Purpose

   PesaR-D promoter in pCS26

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Synth Biol. 2013 Jul 23.

   Insert

   PesaR-D
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Added an additional esa box upstream of -35 site …

   Promoter

   PesaR-D

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pEXP5-NT-AlleyCat

   Plasmid

   #41715

   Depositor

   William DeGrado, Ivan Korendovych

   Article

   Korendovych et al Proc Natl Acad Sci U S A. 2011 Apr 26;10

   Insert

   mutated C-terminal portion of calmodulin gene (M76-K148)
   

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   F92E

   Promoter

   T7

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFredB-PTTG1

   Plasmid

   #16585

   Depositor

   Bert Vogelstein

   Article

   Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

   Insert

   PTTG1 (PTTG1 Human)
   

   Expression

   Mammalian

   Mutation

   3' targeting element

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

5. C119

   Plasmid

   #174354

   Purpose

   CRISPR-Cas9 with guide targeting human PABPC1 genomic location near STOP codon

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   gRNA sequence targeting human PABPC1 near stop codon region
   

   Use

   CRISPR

   Availability

   Academic Institutions and Nonprofits only

   

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6. C205

   Plasmid

   #174359

   Purpose

   For expressing siRNA-resistant frog ePAB in human cell lines via transfection

   Depositor

   David Bartel

   Article

   Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

   Insert

   ePAB
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Multiple silent mutations introduced to be resist…

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT Ptp69D-GFP

   Plasmid

   #126663

   Purpose

   Inducible expression of Ptp69D-GFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Ptp69D
   

   Expression

   Insect

   Mutation

   A53S, K105N, and A98T- please see depositor comme…

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET His10-Draper INT

   Plasmid

   #126670

   Purpose

   Expression of His10-Draper intracellular domain for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic domain
   

   Expression

   Bacterial

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_Gal4UAS_tBFP_PGK_mCherry

   Plasmid

   #79130

   Purpose

   Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

   Depositor

   Wendell Lim

   Article

   Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

   Insert

   tBFP
   

   Use

   Lentiviral

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pimEJ5GFP

    Plasmid

    #44026

    Depositor

    Jeremy Stark

    Article

    Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

    Insert

    EJ5GFP egfp-based chromosomal break reporter
    

    Expression

    Mammalian

    Mutation

    pCAGGS promoter and eGFP separated by pgkPURO cas…

    Promoter

    pCAGGS

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-GroEL/ES-TF

    Plasmid

    #83923

    Purpose

    Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Gro EL/ES
    Trigger Factor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKM492-Myc-His

    Plasmid

    #109283

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-Myc-His
    

    Use

    Orbit integrating plasmid

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-puro largeTcDNA

    Plasmid

    #14088

    Depositor

    William Hahn

    Article

    Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

    Insert

    Large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. mRNA-CleanCap-mNeonGreen

    Plasmid

    #188248

    Purpose

    Template plasmid to transcribe mRNA encoding mNeonGreen

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    mNeonGreen
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. T4 td upstream intron-PABP

    Plasmid

    #189972

    Purpose

    Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    T4 td upstream intron-PABP
    

    Use

    Cloning

    Promoter

    N/A

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMBIS

    Plasmid

    #17817

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Mutation

    D10A

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-gLuc-C1C2

    Plasmid

    #178426

    Purpose

    Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    gLuc
    

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcS-pep1-C1C2

    Plasmid

    #178428

    Purpose

    pep1 as cell penetrating peptide fused to EV membrane binging domain (C1C2)

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    pep1
    

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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