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pdCas9- AsiA_m2.1

Plasmid

#158065

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m2.1

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTOX3

Plasmid

#127448

Purpose

Encodes Tse2 toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEG03

Plasmid

#154138

Purpose

This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

Depositor

Kang Zhou

Article

Panda et al Biochemical Engineering Journal

Insert

fucO, aldA

Expression

Bacterial

Promoter

P_gyrA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pRosa26-DEST

Plasmid

#21189

Purpose

Rosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic mice

Depositor

Nick Hastie, Peter Hohenstein

Article

Hohenstein et al Pathogenetics. 2008 . 1(1):3.

Insert

GateWay conversion cassette

Expression

Mammalian

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Expression

Bacterial

Promoter

J23151

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pVHed05

Plasmid

#82351

Purpose

Contains PBAD-TP901 and Ptet-Bxb1 as well as constitutive TetR

Depositor

Richard Murray

Article

Hsiao et al Mol Syst Biol. 2016 May 17;12(5):869. do

Inserts

TP901-1 integrase
Bxb1 integrase
AraC (araC )
TetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA351-Cpf1

Plasmid

#192361

Purpose

CRISPR-based vector for genome editing

Depositor

Juana María Navarro Llorens

Article

Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

Insert

Cas12a, gRNA scaffold

Use

CRISPR

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRN110

Plasmid

#84462

Purpose

pJB38-NWMN29-30 + SarA_P1-mKate2-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-mKate2 from pRN11

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCasX1

Plasmid

#87685

Purpose

p15A plasmid expressing CasX locus from Deltaproteobacteria

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasX1

Expression

Bacterial

Promoter

None

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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