We narrowed to 16,367 results for: nans

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mVenusN-Cavin1

Plasmid

#108887

Purpose

Expression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

caveolae associated protein 1 (CAVIN1 Human, Synthetic)

Tags

mVenus1-155

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2-mCherry

Plasmid

#140571

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-cPHx1

Plasmid

#116853

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 )

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

Amino acids 169.329

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF39-2xCSTREP

Plasmid

#136200

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF39

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-UPP1_sgRNA1

Plasmid

#201634

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP1 (UPP1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-oScarlet

Plasmid

#137135

Purpose

Viral expression of oScarlet

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

oScarlet

Use

AAV

Mutation

E95D

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37090

Purpose

Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMAL-SH3(2)-5R

Plasmid

#112089

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-5R (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Mutation

construct contains only repeats of the second SH3…

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin1

Plasmid

#108888

Purpose

Expression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin1 (altered codon usage) (CAVIN1 Human, Synthetic)

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4511NES

Plasmid

#138375

Purpose

Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ERK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Puro_siKO-2TO

Plasmid

#86697

Purpose

Tetracycline inducible (2TO version) expression of guide RNA targeted to AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

H1 2TO

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-SC4

Plasmid

#236254

Purpose

AAV-SC4 capsid which targets peripheral nerves in mice

Depositor

Casey Maguire

Article

Abou Haidar et al Gene Ther. 2025 Jun 10. doi: 10.1038/s41

Inserts

rep/cap AAV9 VP1 with GATPSTQ peptide insert
rep

Use

AAV

Expression

Mammalian

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-5HRE-GFP PuroR

Plasmid

#128958

Purpose

Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

5X HRE of VEGF (VEGFA Human)

Use

Lentiviral

Tags

d2EGFP

Expression

Mammalian

Promoter

minimal CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9

Plasmid

#133909

Purpose

Bacterial expression of His6-Ubc9

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS2

Plasmid

#59390

Purpose

Destination vector with RBPMS2 for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131003

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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MTRAP-bio

Plasmid

#47746

Purpose

Expresses enzymatically monobiotinylated full-length MTRAP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MTRAP

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF37-2xCSTREP

Plasmid

#136198

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF37

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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EBA140-bio

Plasmid

#47742

Purpose

Expresses enzymatically monobiotinylated full-length EBA140 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised EBA140

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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