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pDAS12124_PEAR-GFP-preedited

Plasmid

#177179

Purpose

PEAR-GFP control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMT-flp

Plasmid

#67274

Purpose

Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA, flp, repA101, oriR101

Use

Flp recombinase plasmid, temperature sensitive

Tags

His-SUMO (flp)

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pNZ18-CAH

Plasmid

#203159

Purpose

Replicates in E. coli, S. cerevisiae, and A. laidlawii

Depositor

Bogumil Karas

Article

Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

Insert

Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup88

Plasmid

#87338

Purpose

To express human Nup88 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP88 (NUP88 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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hMTP

Plasmid

#124281

Purpose

Expresses human MTP in mammalian cells.

Depositor

Mahmood Hussain

Article

Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

Insert

MTTP (MTTP Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET23a-HsRPS27a

Plasmid

#69561

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
YFP-NavII-III

Plasmid

#26056

Depositor

Matthew Grubb

Article

Grubb et al Nature. 2010 Jun 24. 465(7301):1070-4.

Insert

Nav1.2 (Scn2a Rat)

Tags

YFP

Expression

Mammalian

Mutation

The insert codes only for the intracellular loop …

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pAUC40

Plasmid

#71298

Purpose

Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

Depositor

Susanne von Bodman

Article

Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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p3259 pCMV hUBC9 mt HA

Plasmid

#14439

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 C93S (UBE2I Human)

Tags

HA

Expression

Mammalian

Mutation

Cys93Ser mutation (TGC changed to AGC)

Promoter

CMV

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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SpCas9

Plasmid

#188489

Purpose

Expresses FLAG tagged SpCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-tagged human full length TCF4E pcDNA3

Plasmid

#32738

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

TCF4E (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Expression

Worm

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_2

Plasmid

#164987

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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OPN(-1206)-luc

Plasmid

#31106

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -1206 to +87

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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