We narrowed to 13,467 results for: sequence
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Plasmid#203759PurposeEncodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.DepositorAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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pJBL728
Plasmid#140398PurposeExpresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoterDepositorInsertT7-csoO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL08
Plasmid#218247Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBL716
Plasmid#140386PurposeExpresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoterDepositorInsertT7-mphO-3WJdB-T
UseSynthetic BiologyAvailable SinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJA219= pcDNA3.1V5-completeTRABV-CMVp
Plasmid#116878Purposemammalian expression vector containing TCR sequencesDepositorExpressionMammalianPromoterCMV PromoterAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialPromoterP(rhaB)Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DrFLINC-AKAR-Kras
Plasmid#182334PurposeGenetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.DepositorInsertDrFLINC-AKAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationDronpa contains H193T mutation.PromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2
Plasmid#113945Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
TagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839PromoterCMVAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtTAS1c-B/c
Plasmid#51774PurposeGateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursorDepositorInsertAtTAS1c-B/c
UseGateway-compatible entry cloneMutationA. thaliana TAS1c precursor sequence including a …Available SinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-4mtD3cpv
Plasmid#181874PurposeExpresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoterDepositorInsert4mtD3cpv
UseAAV and AdenoviralExpressionMammalianMutationthe 4mtD3cpv CDS differs from the recorded GeneBa…PromoterCaMKIIaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-Akt-STOPS
Plasmid#175009PurposePlasma membrane-targeted Akt-STOPS.DepositorInsertLyn-mCherry-Akt-STOPS
TagsN-terminal targeting sequence from Lyn kinase and…ExpressionMammalianPromoterCMVAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCCR5-LSSmOrange
Plasmid#110194PurposeEncodes for human CCR5 coding sequence tagged with the LSS-mOrange FPDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUSE-RapR-Src(KD)-myc
Plasmid#25934DepositorInsertRapR-Src (Fkbp1a Mouse)
ExpressionMammalianMutationThis pUSE-RapR-Src(KD)-myc plasmid contains the k…Available SinceOct. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RapR-FAK-YM
Plasmid#25928DepositorInsertRapR-FAK (Ptk2 Human, Mouse)
TagsEGFPExpressionMammalianMutationThis pEGFP-RapR-FAK-YM plasmid contains the FAK m…Available SinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Gal TDP-43 YFP
Plasmid#27468DepositorAvailable SinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-LGC-53
Plasmid#86399PurposeMammalian expression of codon-optimized dopamine receptor LGC-53DepositorInsertLGC-53 (lgc-53 Synthetic, Nematode)
ExpressionMammalianMutationCodon optimized for mammalian expression; Signal …PromoterCMVAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn ChR E90R-D156N-T159C 2A tDimer
Plasmid#52494PurposeHistorical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)DepositorInsertsChannelrhodopsin-2
red fluorescent protein
UseAAVExpressionMammalianMutationE90R, D156N, T159CPromoterhuman synapsin-1 and ribosomal skip sequenceAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-Smad1 (AAVA)
Plasmid#14957DepositorInsertSmad1 (AAVA) (SMAD1 Human)
TagsFlagExpressionMammalianMutationThree Serine to Alanine mutations in the C-termin…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mRuby3-IRES-eGtACR1-ST
Plasmid#108960PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequenceDepositorInserteGtACR1-ST
ExpressionMammalianAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only