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Addgene
Showing: 1681 - 1720 of 2073 results
  1. pSBtet-Bla/mCardinal-TurboID-GAI

    Plasmid
    #198218
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with mCardinal, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Insert
    mCardinal
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. CMYA5tnT/pENTR3C

    Plasmid
    #98129
    Purpose
    CMYA5 (NM_153610, nt 11648 to 12279) T allele (rs10043986) in pENTR3C, Gateway Entry vector
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    rs10043986 (NM_153610.4:c.12188C>T)
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. CMYA5tnC/pDEST26-N-FLAG

    Plasmid
    #98130
    Purpose
    Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. CMYA5tnT/pACT2

    Plasmid
    #98126
    Purpose
    CMYA5 (aa 3859 – 4069) Leu allele (4063) in a GAL4 AD vector, used for yeast-two hybrid
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    rs10043986 (NP_705838.3:p.Pro4063Leu)
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. PML-3/pSG5

    Plasmid
    #50939
    Purpose
    expression of PML with Sumo sites destroyed
    Depositor
    Insert
    PML-3 (PML Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    K65R, K160R, K490R (Sumo modification sites)
    Promoter
    SV40
    Available since
    Jan. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pSCrhaB2plus

    Plasmid
    #164226
    Purpose
    Broad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pSC201plus

    Plasmid
    #164227
    Purpose
    Integrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. CMYA5tnT/pDEST26-N-FLAG

    Plasmid
    #98131
    Purpose
    Express CMYA5 (aa 3859 – 4069) Leu allele (4063) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    rs10043986 (NM_153610.4:c.12188C>T)
    Promoter
    CMV
    Available since
    Aug. 3, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1681 - 1720 of 2073 results