We narrowed to 1,917 results for: FRT

1. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT

   Plasmid

   #204694

   Purpose

   This plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   direct fusion of BRD4 and NUT

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST pcDNA5-FRT/TO-eGFP-Rif1

   Plasmid

   #52506

   Purpose

   mammalian expression vector of Rif1 siRNA resistant

   Depositor

   Daniel Durocher

   Article

   Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

   Insert

   RIF1 (RIF1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: ATAGTAC

   Promoter

   Available Since

   June 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEF5B-FRT-AP-Sstr3-GFP-DEST

   Plasmid

   #49098

   Purpose

   Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

   Depositor

   Maxence Nachury

   Article

   Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

   Insert

   Sstr3 (Sstr3 Mouse)
   

   Use

   Tags

   GFP and acceptor peptide (AP)

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-WT

   Plasmid

   #218834

   Purpose

   Expresses N-terminal BirA*-tagged human wild type TLK1 variant 5 for BioID experiments.

   Depositor

   Travis Stracker

   Insert

   TLK1 (TLK1 Human)
   

   Use

   Tags

   FLAG-BirA*

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TetO-U2B''-fSNAPc

   Plasmid

   #106988

   Purpose

   open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   UB2" (SNRPB2 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TetO-U1-70K-fSNAPc

   Plasmid

   #106987

   Purpose

   open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   U1-70K (SNRNP70 Human)
   

   Use

   Tags

   fSNAP

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO_Vim-SNAP-tag2

   Plasmid

   #226518

   Purpose

   Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   Vimentin-SNAP-tag2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9

   Plasmid

   #175539

   Purpose

   Expression of HaloTag9 at the inner membrane of mitochondria in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Cox8a-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-FRT-Rev-3xGFP

   Plasmid

   #191204

   Purpose

   FlpO dependent GFP expression

   Depositor

   Janelia Viral Tools

   Article

   Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

   Insert

   3X GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-FRT-Rev-TdTomato

    Plasmid

    #191203

    Purpose

    FlpO dependent TdTomato expression

    Depositor

    Janelia Viral Tools

    Article

    Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

    Insert

    TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5/FRT/DEST-3xHA/mGli2

    Plasmid

    #51250

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

    Plasmid

    #51251

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 789,805,817,848,923,956 mutated to Ala

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pEF5B-FRT-GFP-αTAT1[D157N]

    Plasmid

    #27100

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Use

    Tags

    GFP and S-tag

    Expression

    Mammalian

    Mutation

    D157N

    Promoter

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA5/FRT/TO-[4xCox8]-ChemoG-NAD

    Plasmid

    #193823

    Purpose

    CMV overexpression of ChemoG-NAD in the mitochondria of mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    [4xCox8]-ChemoG-NAD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST 3x Flag-pcDNA5-FRT/T0-BRCA1

    Plasmid

    #52504

    Purpose

    mammalian expression vector of BRCA1 wildtype siRNA resistant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: AGTATAATC

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pFRT-TO-RPB1-His WT CRres si2,4R

    Plasmid

    #139404

    Purpose

    Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV, TRex

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5-FRT-V5-DEST-GFP-Kif26b

    Plasmid

    #102862

    Purpose

    Expresses GFP-Kif26b in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    Mouse Kif26b (Kif26b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

    Plasmid

    #189792

    Purpose

    Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

    Depositor

    Robert Singer

    Article

    Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

    Insert

    beta-globin (HBB Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ponasterone A inducible

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5 frt/to N-BioTAP-C-BRD4-NUT

    Plasmid

    #171630

    Purpose

    Expresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.

    Depositor

    Christopher A French, Chris French

    Article

    Alekseyenko et al Proc Natl Acad Sci U S A. 2017 May 23;11

    Insert

    BRD4-NUT (BRD4, NUTM1 Human)
    

    Use

    Tags

    BioTAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF5B-FRT-AP-Smo-YFP-DEST

    Plasmid

    #49099

    Purpose

    Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

    Depositor

    Maxence Nachury

    Article

    Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    YFP and acceptor peptide (AP)

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Nov. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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