Addgene

We narrowed to 2,388 results for: MEA;

Showing: 841 - 860 of 2388 results

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pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

Plasmid

#160741

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Use

Tags

mGFP and mScarlet

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_FOXP1_1

Plasmid

#86299

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_2

Plasmid

#86308

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_FOXP1_2

Plasmid

#86300

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pOPS0380

Plasmid

#133230

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_CEBPG

Plasmid

#86285

Purpose

Donor vector for 3' FLAG tag of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CEBPG homology arms (CEBPG Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_GATAD2A

Plasmid

#86279

Purpose

Donor vector for 3' FLAG tag of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GATAD2A homology arms (GATAD2A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_MED13_iso1

Plasmid

#135733

Purpose

Donor vector for 3' FLAG tag of human MED13_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MED13_iso1 Homology arms (MED13 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMA3379

Plasmid

#46874

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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PX458_ARID5B_1

Plasmid

#86350

Purpose

Encodes gRNA for 3' target of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID5B (ARID5B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPA_1

Plasmid

#86358

Purpose

Encodes gRNA for 3' target of human CEBPA

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against CEBPA (CEBPA Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_BCL6_iso1_2

Plasmid

#104047

Purpose

Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 gRNA (BCL6 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_BCL6_iso1_1

Plasmid

#104046

Purpose

Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 gRNA (BCL6 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_BCL6_iso1

Plasmid

#104038

Purpose

Donor vector for 3' FLAG tag of human BCL6_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

BCL6_iso1 homology arms (BCL6 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMA3383

Plasmid

#46875

Purpose

A retroviral vector for the constitutive expression of soluble guanylate cyclase1 beta3

Depositor

Mikhail Alexeyev, Troy Stevens

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1beta 3 (Gucy1b3 Rat)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

Aug. 13, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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PX458_ARID5B_2

Plasmid

#86351

Purpose

Encodes gRNA for 3' target of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ARID5B (ARID5B Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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