We narrowed to 7,670 results for: URE

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pFH2.203_PB-EPN24-MCP-IRES-eGFP-PGK-Blast

Plasmid

#205555

Purpose

Express EPN24-MCP-IRES-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-Dendra2

Plasmid

#82436

Purpose

Dendra2 expression plasmid

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2

Tags

GST tag

Expression

Bacterial

Promoter

tac

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pAYP (pDre)

Plasmid

#62709

Purpose

Mammalian expression of Dre recombinase from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

Dre

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-10XHis-GST-HRV-dL5

Plasmid

#73214

Purpose

Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

Depositor

Marcel Bruchez

Article

Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

Insert

EGFR (EGFR Schistosoma japonicum, Human)

Tags

10XHis-GST-HRV

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pHR iRFP-ZtSH2

Plasmid

#188627

Purpose

Fluorescent reporter for CD3ε/ZtSH2 pYtag biosensor

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

ZtSH2

Use

Lentiviral

Tags

iRFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJYDC1

Plasmid

#162458

Purpose

Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

HA tag, HDEL sequence, eUnaG2 fluorescent protein…

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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GCEPIA1-SNAPER

Plasmid

#172659

Purpose

Detect the real-time ER calcium store and local calcium microdomains in live cells.

Depositor

Pingyong Xu

Article

Luo et al Biophys Rep (2019) 5, 31–42

Insert

calmodulin

Expression

Mammalian

Mutation

Please see depositor comments

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-OMM-GCaMP6f

Plasmid

#127874

Purpose

Fluorescent reporter for mitochondrial outer membrane Ca2+

Depositor

Timothy Ryan

Article

Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

Insert

Outer membrane localized GCaMP6f

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT5

Plasmid

#53556

Purpose

mammalian expression of IFIT5

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT5 (IFIT5 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1D V5-His-TOPO-KGA.wt

Plasmid

#110332

Purpose

Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

KGA glutaminase (GLS Human)

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff EYFP

Plasmid

#55651

Purpose

Cre-on/Flp-off EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre on/flp off eyfp

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Plasmid

#115684

Purpose

NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

Depositor

David Suter

Article

Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

Insert

Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Use

Lentiviral

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTfR-mNeonGreen

Plasmid

#129608

Purpose

In vivo visualization of Transferrin receptors (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Transferrin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Klentaq

Plasmid

#157862

Purpose

Plasmid for the aTc-inducible expression of the Klentaq

Depositor

Andrew Ellington

Article

Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

Insert

Klentaq

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

Plasmid

#176836

Purpose

Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP

Use

Unspecified

Mutation

See Depositor Comments Below

Promoter

Tet operator (tetracycline inducible expression)

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo-hCG

Plasmid

#16574

Depositor

Bert Vogelstein

Article

Shih et al Nat Med. 2000 Jun . 6(6):711-4.

Insert

Beta-hCG (CGB3 Human)

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-mWnt11

Plasmid

#42290

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt 11 (Wnt11 Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac-His-MBP-SCov2-nsp12

Plasmid

#154759

Purpose

Expression of SARS-CoV-2 nsp12

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Insect

Promoter

Polyhedron

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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