We narrowed to 313,530 results for: addgene

1. XLone-eGFP NLS (no drug resistant gene)

   Plasmid

   #140032

   Purpose

   Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7M3X

   Plasmid

   #176411

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   RBBP7 (RBBP7 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   Oct. 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. plenti guide ds red

   Plasmid

   #128055

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6NM4

   Plasmid

   #162257

   Purpose

   Bacterial Expression

   Depositor

   Cheryl Arrowsmith

   Insert

   PRDM9 (PRDM9 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. 5KKP (PUS7)

   Plasmid

   #101878

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PUS7 (PUS7 Human)
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. TDRD3 (3PMT)

   Plasmid

   #28274

   Depositor

   Cheryl Arrowsmith

   Insert

   Tudor domain-containing protein 3 (TDRD3 Human)
   

   Tags

   His with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains Tudor domain, residues 553-611.

   Available Since

   April 14, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

7. SpCas9_hGEM(1-110)_hSLBP(56-108)

   Plasmid

   #191388

   Purpose

   SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_hSLBP(56_108)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pINDUCER20-hMYF5-3xHA (#732)

   Plasmid

   #78332

   Purpose

   Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag

   Depositor

   Louis Kunkel, Matthew Alexander

   Insert

   MYF5 (MYF5 Human)
   

   Use

   Lentiviral

   Tags

   3x HA tag

   Promoter

   TRE2

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

9. MIGR1-Meis1a

   Plasmid

   #78148

   Purpose

   retroviral expression of Meis1a and GFP

   Depositor

   Francesco Blasi

   Insert

   Meis1a (Meis1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

10. 5TF2 (PREB)

    Plasmid

    #101889

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PREB (PREB Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis, TEV

    Promoter

    polyhedrin

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. NUDT7 (NUDT7A-c002)

    Plasmid

    #98235

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NUDT7A (NUDT7 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid

    #104412

    Purpose

    transient expression of dCas9-dDNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    dDNMT3a
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    C706S

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAGEA3

    Plasmid

    #73246

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAGEA3 (MAGEA3 Human)
    

    Tags

    His6-Thioredoxin-TEV

    Expression

    Bacterial

    Mutation

    pfam01454:MAGE 116-286

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDK714

    Plasmid

    #134230

    Purpose

    Mammalian expresion of tdTomato SKAP

    Depositor

    Iain Cheeseman

    Insert

    DK365 tdTomato (short SKAP) (KNSTRN Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid

    #135668

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgCR8
    

    Use

    Mouse Targeting

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4RG1

    Plasmid

    #64657

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    C9orf114:JMC099-C04:C232626 (SPOUT1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    May 15, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pLV CDHblast OST-Myorg-V5

    Plasmid

    #175171

    Purpose

    Lentiviral expression of mouse Myorg-V5

    Depositor

    Larry Gerace

    Insert

    Myorg (Myorg Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV CDHblast OST-Myorg(D462A)-V5

    Plasmid

    #175172

    Purpose

    Lentiviral expression of mouse Myorg-V5

    Depositor

    Larry Gerace

    Insert

    Myorg (Myorg Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    D462A

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pINDUCER20-FLAG-hMYF5 (#801)

    Plasmid

    #78334

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYF5 (MYF5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. 4TVR

    Plasmid

    #61952

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UBH14 (UHRF2 Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits