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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 159,596 results for: addgene

Showing: 841 - 860 of 159596 results

  1. pHR_SFFV-sig-mCherry-PDGFRTM-WPRE

    Plasmid
    #216668
    Purpose
    Expresses membrane-bound mCherry in mammalian cells
    Depositor
    Leonardo Morsut
    Insert
    mCherry
    Use
    Tags
    PDGFR Transmembrane Domain
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216668-plasmid-map-sequence-id-432187

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  2. p14-9VN

    Plasmid
    #50930
    Purpose
    Expresses human SRP14 and SRP9 proteins as a fusion protein in mammalian cells.
    Depositor
    Katharina Strub
    Insert
    SRP14 and SRP9 (SRP14, SRP9 Human)
    Use
    Tags
    VN tag and flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50930-plasmid-map-sequence-id-422738

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  3. SRPK1 (SRPK1A-c100)

    Plasmid
    #98246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK1A (SRPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98246-plasmid-map-sequence-id-193287

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  4. pHR_EF1a_flagLaM4-Notch-Gal4VP64

    Plasmid
    #216669
    Purpose
    Expresses anti-mCherry synNotch Gal4VP64
    Depositor
    Leonardo Morsut
    Insert
    anti-mCherry synNotch Gal4VP64
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    216669-plasmid-map-sequence-id-432194

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  5. AAVS1-SAGFP

    Plasmid
    #127100
    Purpose
    SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.
    Depositor
    Adam Karpf
    Insert
    SA-GFP reporter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127100-plasmid-map-sequence-id-247555

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  6. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140032-plasmid-map-sequence-id-275878

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  7. 7M3X

    Plasmid
    #176411
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    RBBP7 (RBBP7 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    176411-plasmid-map-sequence-id-345851

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  8. plenti guide ds red

    Plasmid
    #128055
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128055-plasmid-map-sequence-id-255092

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  9. 6NM4

    Plasmid
    #162257
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM9 (PRDM9 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162257-plasmid-map-sequence-id-319826

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  10. 5KKP (PUS7)

    Plasmid
    #101878
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PUS7 (PUS7 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101878-plasmid-map-sequence-id-194704

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  11. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

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  12. integrin beta6 pBabe puro

    Plasmid
    #13596
    Depositor
    Dean Sheppard
    Insert
    integrin beta 6 (ITGB6 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 8, 2007
    Availability
    Academic Institutions and Nonprofits only

  13. TDRD3 (3PMT)

    Plasmid
    #28274
    Depositor
    Cheryl Arrowsmith
    Insert
    Tudor domain-containing protein 3 (TDRD3 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Tudor domain, residues 553-611.
    Promoter
    Available since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pINDUCER20-hMYF5-3xHA (#732)

    Plasmid
    #78332
    Purpose
    Doxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYF5 (MYF5 Human)
    Use
    Lentiviral
    Tags
    3x HA tag
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. MIGR1-Meis1a

    Plasmid
    #78148
    Purpose
    retroviral expression of Meis1a and GFP
    Depositor
    Francesco Blasi
    Insert
    Meis1a (Meis1 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. NUDT7 (NUDT7A-c002)

    Plasmid
    #98235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT7A (NUDT7 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98235-plasmid-map-sequence-id-193415

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  17. 5TF2 (PREB)

    Plasmid
    #101889
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PREB (PREB Human)
    Use
    Baculovirus expression
    Tags
    6xHis, TEV
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101889-plasmid-map-sequence-id-194722

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  18. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid
    #104412
    Purpose
    transient expression of dCas9-dDNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    dDNMT3a
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    C706S
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104412-plasmid-map-sequence-id-204224

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  19. pIC301

    Plasmid
    #69732
    Purpose
    Retroviral expression of Bub1
    Depositor
    Iain Cheeseman
    Insert
    Bub1 (BUB1 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. MAGEA3

    Plasmid
    #73246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA3 (MAGEA3 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    pfam01454:MAGE 116-286
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73246-plasmid-map-sequence-id-137200

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Showing: 841 - 860 of 159596 results