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172,845 results

Showing: 8581 - 8600 of 172845 results

  8. pOT_5 - lenti-EFS-FMC6.3-28z-2A-puro-2A-tNGFR

    Plasmid
    #181974
    Purpose
    Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.
    Depositor
    Neville Sanjana
    Article
    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:
    Insert
    FMC6.3-28z CAR; tNGFR (NGFR Synthetic, Human)
    Use
    Lentiviral
    Mutation
    NGFR truncation: aa 11-276
    Available Since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    181974-plasmid-map-sequence-id-355578

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  9. MSP2133

    Plasmid
    #72249
    Purpose
    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    Y450A, N497A, R661A, Q695A, and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72249-plasmid-map-sequence-id-131869

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  10. pBOB-VE-cadherin-tdTomato

    Plasmid
    #118733
    Purpose
    To express VE-cadherin tagged with tdTomato on C-terminus
    Depositor
    John Cooper
    Insert
    Cadherin 5 (CD144) (CDH5 Human)
    Use
    Lentiviral
    Tags
    tdTomato
    Mutation
    I503T (Common SNP-see comments)
    Promoter
    CMV
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    118733-plasmid-map-sequence-id-229276

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  11. CMV-eGFP-Z1

    Plasmid
    #135049
    Purpose
    Enhanced Green fluorescent protein expression for mammalian cells under constitutive CMV Promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available Since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135049-plasmid-map-sequence-id-269408

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  15. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187952-plasmid-map-sequence-id-373488

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  16. AAV-EF1a-jGCaMP8f-WPRE (AAV9)

    Viral Prep
    #176756-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-EF1a-jGCaMP8f-WPRE (#176756). In addition to the viral particles, you will also receive purified AAV-EF1a-jGCaMP8f-WPRE plasmid DNA. EF1a-driven expression of calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    EF1a
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  20. pAAV.GFA104.PI.eGFP.WPRE.bGH (AAV5)

    Viral Prep
    #100896-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GFA104.PI.eGFP.WPRE.bGH (#100896). In addition to the viral particles, you will also receive purified pAAV.GFA104.PI.eGFP.WPRE.bGH plasmid DNA. GFA104-driven eGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Philip Haydon
    Article
    Ortinski et al Nat Neurosci. 2010 May;13(5):584-91. doi
    Promoter
    GFA104 (GFAP promoter)
    Tags
    EGFP
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 8581 - 8600 of 172845 results