We narrowed to 19,452 results for: Met;

Showing: 8581 - 8600 of 19452 results

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hSyn-PostSynBar-hSyn-PostRNA short

Plasmid

#247716

Purpose

SynBar protein and RNA construct used in HEK and neuron culture experiment.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

PostSynBar and PostRNA short

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL1-FLAG

Plasmid

#15818

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin1 (CUL1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N452

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Expression

Bacterial

Mutation

copFnull

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Mmp11

Plasmid

#209520

Purpose

Expresses luciferase under control of Mmp11 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

Insert

Mmp11 promoter (Mmp11 Mouse)

Use

Luciferase

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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#1.4 post-R-SynapShot-Lyn-miRFP670

Plasmid

#214718

Purpose

Expresses red #1.4 post-SynapShot and Lyn-miRFP670

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-R-SynapShot

Tags

Lyn-miRFP670

Expression

Mammalian

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMB34

Plasmid

#122245

Purpose

Lentivirus delivery for stable expression of LbCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670nano3

Plasmid

#184663

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano3 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Tags

His

Expression

Bacterial

Promoter

araC

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

Plasmid

#104119

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Adenoviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL2-FLAG

Plasmid

#15819

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin2 (CUL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N427

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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Dendra2-Actin-C-18

Plasmid

#57701

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p330 VQR

Plasmid

#101730

Purpose

Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMP11

Plasmid

#215559

Purpose

pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Inserts

Cas9
gRNA targeting pBR322 origin of replication

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23105 and tet

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Twin-Strep(R)-Tev-GSGS-Ubiquitin

Plasmid

#186803

Purpose

Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Twin-Strep(R)-Tev-GSGS-Ubiquitin

Tags

GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

Expression

Bacterial

Mutation

Strep tag Lysine's mutated to Arginine

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCN-mBFP

Plasmid

#63847

Purpose

Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.

Depositor

Howard Salis

Article

Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

Insert

mBFP

Expression

Bacterial

Mutation

Promoter and RBS was swapped. His-tag was removed…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTK82_GFP-4.1G

Plasmid

#46353

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G (EPB41L2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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Dimer cleaved

Plasmid

#198199

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-T2A-mTurquoise2

Expression

Mammalian

Mutation

A206K both

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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