We narrowed to 42,102 results for: Eras
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Plasmid#219747PurposeDVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expressionDepositorInsertpCMV - nnLuz_v4 - tSV40
UseLuciferaseExpressionMammalianAvailable SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hfnCas12a_CRATES
Plasmid#176251PurposepNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and type IIS restriction site for endonuclease BaeI at the crRNA region that facilitates the generation of mulitplDepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIZ-EGFP-MYO10-HMM
Plasmid#87257PurposeExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionInsectMutationMyo10 is truncated to contain aa.1-941PromoterOpIE2Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1092-luc2
Plasmid#227112PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-mRFP-; HE1A:BFP -1
Plasmid#180008PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-mRFP; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBJG1 T7-8xHis-OGT K842M
Plasmid#154272PurposeBacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags8x His, HRV3C protease site, and T7 leaderExpressionBacterialMutationLysine 842 changed to Methionine- bacterial codon…Available SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM19
Plasmid#123655PurposeEncodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cellsDepositorInsertFirefly luciferase
UseLuciferaseExpressionInsectPromoterpIExAvailable SinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-eIF4G delN83
Plasmid#65812Purposeexpression of eIF4G-MS2 fusion.DepositorInserteIF4G (EIF4G1 Human)
TagsMS2 stem loops and V5ExpressionMammalianMutationdelN83 (functionally behaves as the WT protein)PromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-42)
Plasmid#127151PurposeExpresses N-terminal cysteine Abeta(1-42)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP
Plasmid#189630PurposeExpresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV, Cre/Lox, and LuciferaseTagsGluc and IgG1 FcExpressionMammalianPromoterIRES and hSynAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-ChR2-YFP
Plasmid#204345PurposePiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.DepositorInsertChR2-YFP
TagsYellow fluorescent proteinExpressionMammalianMutationC-terminus (aa315-737) is deleted - PMID: 1461559…PromoterCAGAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3 1 kb prom and exon1 fragment CD274
Plasmid#107006PurposeModified Luciferase expression vectorDepositorAvailable SinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO10-HMM
Plasmid#87256PurposeExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2KXIG (Tol2)-tp63:ikk1KD:GFP
Plasmid#105645PurposeKinase-deleted Ikk1(alpha) expression in basal keratinocytesDepositorInserttp63:ikk1KD:GFP (chuk Zebrafish)
TagsGFPExpressionMammalianMutationp63 promoter + ikk1 (kinase domain deleted) + GFPAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL11060
Plasmid#68264PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)DepositorInsertPromoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
UseSynthetic BiologyExpressionPlantAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO-T2A-Cre LV
Plasmid#202003PurposeGenerates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP miceDepositorInsertHELLO-T2A-Cre
UseLentiviralTagsFLAGPromoterpGKAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Flag-Jun4A-Myc
Plasmid#47444Purposemammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)DepositorInsertJun 4A mutant (Jun Mouse)
TagsFlag and mycExpressionMammalianMutationfour N-terminal residues phosphorylated by JNK, s…PromoterCMVAvailable SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1014-luc2
Plasmid#227108PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1014DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1014 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; HE1A:BFP -0
Plasmid#180010PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_ER-RE_MLPmin_BC1063-luc2
Plasmid#227109PurposeEstrogen receptor response element for luciferase and barcode assays; barcode BC1063DepositorInsert12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only