We narrowed to 14,509 results for: SHI

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pMito-miRFP718nano

Plasmid

#195754

Purpose

Mitochondria labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

Human cytochrome C oxidase subunit VIII-miRFP718nano

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro Myr HA PIK3CB

Plasmid

#12527

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele.

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

Plasmid

#197067

Purpose

Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

MitoTrap-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCRU5-inmCherry

Plasmid

#60238

Purpose

HTLV-1 based transfer vector encoding mcherry reporter gene interrupted by intron

Depositor

Dmitriy Mazurov

Article

Shunaeva et al J Virol. 2015 Oct 15;89(20):10591-601. d

Insert

inmCherry

Use

Retroviral

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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HA-VHL W117R-pBabe-puro

Plasmid

#19239

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tryptophan 117 to Arginin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pTorPE-G-GECO1.2

Plasmid

#32468

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1

Plasmid

#184566

Purpose

Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Expression

Bacterial

Promoter

T7 promoter

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pFB-HTB-ASK1

Plasmid

#29517

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

ASK1 (SKP1 Mustard Weed)

Expression

Insect

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
AsCas12a-DR-cr in PBCSK-

Plasmid

#126640

Purpose

Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

AsCas12a Full Length Direct Repeat crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP718nano

Plasmid

#195750

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

H2B-miRFP718nano

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-sh-mSlug-3

Plasmid

#40647

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-mSlug-3 (Snai2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
gh39

Plasmid

#106713

Purpose

expression of gRNA targeting STT3B

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

STT3B (STT3B Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh171

Plasmid

#106832

Purpose

expression of gRNA targeting UGCG

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

UGCG (UGCG Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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p5077 pcDNA4c hBrd4 (1-594)

Plasmid

#14453

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-594) (BRD4 Human)

Tags

His

Expression

Mammalian

Mutation

amino acids 1-594

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pNP1 eforRed toehold switch

Plasmid

#107353

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 1 + eforRed

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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FOR human NANOS3 TALEN

Plasmid

#59722

Purpose

TALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

HD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG

Use

TALEN

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only