We narrowed to 12,230 results for: nsf

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pAAV asyn S87A

Plasmid

#36056

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein S87A (SNCA Human)

Use

AAV

Expression

Mammalian

Mutation

S87A

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pAAV CB6 FFLuc-miR122

Plasmid

#35656

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

pri-miR-122 (Mir122 )

Use

AAV

Promoter

CB

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 ChR2 ET-TC 2A tDimer

Plasmid

#101361

Purpose

High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
synaptophysin-red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E123T , T159C

Promoter

human Synapsin

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Axin1_HA in pRK5

Plasmid

#154220

Purpose

C-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

AXIN1 (AXIN1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-mGrn-myc

Plasmid

#178944

Purpose

Construct for expressing murine progranulin (Grn) in AAV

Depositor

Erik Roberson

Article

Arrant et al Brain. 2017 May 1;140(5):1447-1465. doi:

Insert

Grn (Grn Mouse)

Use

AAV

Tags

Myc

Promoter

CBA

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Zeo-TTF-1

Plasmid

#75089

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

TTF-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-erHRP(N175S mutant)

Plasmid

#79906

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, erHRP(N175S mutant)

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

erHRP(N175S mutant)

Use

AAV and Cre/Lox

Tags

HA tag

Expression

Mammalian

Mutation

N175S mutation

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJEP301-pAAV-CMV-MCS3-pA

Plasmid

#113676

Purpose

CMV driven Multi Cloning Site-3

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSKA397

Plasmid

#80380

Purpose

mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

cat
metE

Use

Synthetic Biology; Mini-tn7 delivery vector

Expression

Bacterial

Promoter

cat promoter and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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MSP7-bio

Plasmid

#47735

Purpose

Expresses enzymatically monobiotinylated full-length MSP7 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MSP7

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV_12xTEAD_luc2

Plasmid

#173004

Purpose

firefly luciferase reporter vector with 12x clustered TEAD binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

12xTEAD-luc2

Use

AAV and Luciferase

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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CSAC-Bmal1

Plasmid

#168296

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgBmal1-1-hU6-sgBmal1-3

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eYFP-3x-miR708-5p-TS

Plasmid

#117381

Purpose

An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

Plasmid

#184633

Purpose

Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Aldh1a1 (Aldh1a1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChromeQ-GFP

Plasmid

#123317

Purpose

AAV-mediated expression of ChromeQ-GFP under the Syn promoter.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

Syn

Available Since

March 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125236

Purpose

Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAC8_GWs-Lox (VE5589)

Plasmid

#163769

Purpose

Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

Depositor

Arnaud Poterszman

Article

Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

Insert

no insert

Tags

no tag

Expression

Insect

Promoter

PH

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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MSP5-bio

Plasmid

#47718

Purpose

Expresses enzymatically monobiotinylated full-length MSP5 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised MSP5

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

Plasmid

#37089

Purpose

Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-off

Tags

TdTomato

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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