We narrowed to 16,572 results for: nans

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Gluc-LHCGR

Plasmid

#221781

Purpose

Measurement of glycoprotein-glycoprotein receptor binding using the BRET phenomenon and measurement of membrane expression of the Gluc-LHCGR

Depositor

Adolfo Rivero-Muller

Article

Adamczuk et al Endocrinology. 2024 Apr 29;165(6):bqae05

Insert

LHCGR (LHCGR Human)

Tags

Gaussia Luciferase and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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RH1-bio

Plasmid

#47748

Purpose

Expresses enzymatically monobiotinylated partial RH1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RH1 (partial)

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-pDEST_BirA_Flag

Plasmid

#227960

Purpose

BioID experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

BirA

Expression

Mammalian

Mutation

R118G

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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TRBV5-1

Plasmid

#123414

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV5-1 (TRBV5-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF73-2xCSTREP

Plasmid

#136232

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF73

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF73

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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β₂AR-mCFP

Plasmid

#38260

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

β₂AR (ADRB2 Human)

Tags

Flag, mCFP, and signal peptide sequence (MKTIIALS…

Expression

Mammalian

Mutation

diglycine linker (replacing terminator codon)

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

Plasmid

#35501

Purpose

AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

Depositor

Karl Deisseroth

Article

Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

C128S and D156A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-DIO-mCReP

Plasmid

#167503

Purpose

Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

Ppp1r15b (Ppp1r15b Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

hSyn

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Coff/Fon-EYFP

Plasmid

#231927

Purpose

Cre-off/Flp-on EYFP under the Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV

Promoter

Ef1a

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-NLS-TPP1

Plasmid

#53585

Purpose

Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (ACD Human)

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Silent mutation to eliminate PacI site

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
TRBV20-1

Plasmid

#123454

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV20-1 (TRBV20-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-C (LEU2)

Plasmid

#177796

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[3]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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TPC2-D276K-G-GECO1.2

Plasmid

#207142

Purpose

Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

TPC2N2 (TPCN2 Human)

Tags

G-GECO1.2

Expression

Mammalian

Mutation

Changed Aspartate 276 to Lysine

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF50-2xCSTREP

Plasmid

#136211

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF50

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K5-2xCSTREP

Plasmid

#136154

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K5

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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Hygro-iNGN3

Plasmid

#75340

Purpose

doxycycline inducible NGN3 gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

NGN3 (NEUROG3 Human)

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRmine-eYFP-WPRE

Plasmid

#130992

Purpose

AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

eYFP

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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TagBFP2-INPP4B-CAAX

Plasmid

#116857

Purpose

plasma membrane-targetted PI(3,4)P2 4-phosphatase

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

INPP4B (INPP4B Human)

Tags

HRAS(172-189) and TagBFP2

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

Plasmid

#194231

Purpose

Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

EF1a-LCB1-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pWPI-UPP1-3xFLAG

Plasmid

#201644

Purpose

Over-expression of 3xFLAG-tagged human UPP1

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP1 (UPP1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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