We narrowed to 10,808 results for: T7

Showing: 8721 - 8740 of 10808 results

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PET-Blackjack SpCas9-NLS-6xHis

Plasmid

#207375

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Blackjack SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

amino acids 1005-1013 replaced with two glycine

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 sf:em-blue-shift.1

Plasmid

#191928

Purpose

Encodes a GFP design, sf:em-blue-shift.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:em-blue-shift.1

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69P S72A T108V Y145M T167V V224I

Promoter

T7

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL2 residues 1-98

Plasmid

#180619

Purpose

Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL2 (KATNAL2 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-98

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KC1 MIT, residues 235-316

Plasmid

#180622

Purpose

Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KC1 (RPS6KC1 Human)

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 235-316

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_beta-Villin

Plasmid

#200301

Purpose

Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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H6GB1tev-IntC-P53(304-393)

Plasmid

#200315

Purpose

bacterial expression of P53 TETCTD for segmental labeling

Depositor

Peter Wright

Article

Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

Insert

p53 (304-393) (TP53 Human)

Tags

His6GB1tev and designed intein C

Expression

Bacterial

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET32a-hNRG1a

Plasmid

#199235

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

Insert

Neuregulin (NRG1 Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W5F

Plasmid

#198443

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W5F

Promoter

T7 promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W89F

Plasmid

#198447

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W89F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W83F

Plasmid

#198446

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W83F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W69F

Plasmid

#198445

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W69F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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eZiPro

Plasmid

#198434

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

enzymatically cleavable linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro W50F

Plasmid

#198444

Purpose

Activity/inhibition assays, 19F-NMR assignment

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A, W50F

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCasΔ4_1_2_VA

Plasmid

#186440

Purpose

Encodes Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b

Plasmid

#186449

Purpose

Encodes Cas12b from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 sf:em-blue-shift.2

Plasmid

#191919

Purpose

Encodes a GFP design, sf:em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:em-blue-shift.2

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 em-blue-shift.2

Plasmid

#191901

Purpose

Encodes a GFP design, em-blue-shift.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

em-blue-shift.2

Tags

His Tag

Expression

Bacterial

Mutation

T65S S72A T108I Y145F V150I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.2

Plasmid

#191894

Purpose

Encodes a GFP design, slow.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.2

Tags

His Tag

Expression

Bacterial

Mutation

V68M Q69L S72T V112I Y145M T167V L220V V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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