We narrowed to 8,836 results for: construct
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Plasmid#124258PurposeExpresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.DepositorInsertshATRX
ExpressionMammalianAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQ-Elo-HB-C-A(400-772)
Plasmid#171087PurposeCo-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOBDepositorTagsHisExpressionBacterialMutationDeleted N-term 26+399 amino acids for mutant expr…PromoterT5 promoter and T7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Myc(Δ1-143; ΔDeg1ΔDeg2)-GFP-IRES-mCherry (ΔTADΔDeg1ΔDeg2)
Plasmid#231234PurposeMYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and point mutations corresponding to the deletion of degron 1 and 2 for transient expression in mammalian cellsDepositorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
px330 Gatad2a sgRNA (for flox targeting)
Plasmid#110815PurposeUsed with pNTK Gatad2a flox allele targeting construct in mouse cellsDepositorInsertmGataD2a (Gatad2a Mouse)
ExpressionMammalianAvailable SinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
EXPRESSYALI
Plasmid Kit#1000000245PurposeAssembly of expression constructs for Yarrowia lipolytica.DepositorApplicationCloning and Synthetic BiologyVector TypeYeast ExpressionCloning TypeGolden GateAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-UbC-rtTA-Ngn2:2A:Ascl1
Plasmid#127289Purposeall-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)DepositorUseLentiviralTagsNgn2 and Ascl1 linked via 2AExpressionMammalianAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_S5E2_Chrimson_tdTomato
Plasmid#219432PurposeAAV construct expressing Chrimson-tdTomato under the control of E2 regulatory elementDepositorInsertChrimson-tdTomato
UseAAVAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
delta PE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52380PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsExpressionMammalianMutationdeleted -1620 - -1011 upstream from OCT4 ATGAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
mouse deltaPE Oct4-GFP transgenic reporter
Plasmid#52382PurposeBAC recombineering generated construct, used as a reporter for mouse Oct4 expressionDepositorInsertsExpressionMammalianMutationdeleted -300 - -1300bp fragment upstream from Oct…PromoterOct4 and PGK+gb2Available SinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
delta DE OCT4-GFP-2A-PURO transgenic reporter
Plasmid#52381PurposeBAC recombineering generated construct, used as a reporter for OCT4 expressionDepositorInsertsExpressionMammalianMutationdeleted -3194 - -1768 upstream from OCT4 ATGPromoterOCT4Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMC00027
Plasmid#199279PurposeSpCas9 construct to knockout murine Cd274DepositorAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Endy/Shearwin pOSIP plasmid set
Plasmid Kit#1000000035PurposeCloning and assembly of DNA constructs for chromosomal integration in E. coli.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeClonetegrationAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
MultiSite Gateway plasmid toolKit
Plasmid Kit#1000000050PurposePlasmids for constructing fully-customizable multi-part fusion proteins.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial Expression, Eukaryotic Expression, Mammalian ExpressionCloning TypeMultiSite GatewayAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
OVC Extended Collection
Plasmid Kit#1000000165PurposeExpansion kit for #1000000164 includes alternative linker configurations in site B and light-activated Opto-caspase 9 constructs.DepositorApplicationCloning and Synthetic Biology, Gene Expression and LabelingVector TypeMammalian ExpressionCloning TypeRestriction EnzymeAvailable SinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-12)
Plasmid#219420PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-12)
TagsMGHHHHHGGExpressionBacterialPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-LSSmCherry1(-13)
Plasmid#219415PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertLSSmCherry1(-13)
TagsMGHHHHHGGExpressionBacterialMutationK137E, K199EPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-7)-neg_tag1
Plasmid#219419PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-7)-neg_tag1
TagsDEEEDD and MGHHHHHGGExpressionBacterialPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mBlueberry(-9)
Plasmid#219416PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmBlueberry2(-9)
TagsMGHHHHHGGExpressionBacterialMutationK137E, K199E, R234EPromoterT7Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-mScarlet-I(+5)
Plasmid#219418PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertmScarlet-I(+5)
TagsMGHHHHHGGExpressionBacterialMutationD125K, D186K, E216K, E238KPromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-a(+)-6xHis-miRFP(-5)-HO1
Plasmid#219424PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertsmiRFP682
HO1
TagsMGHHHHHGGExpressionBacterialMutationContains Ribosomal Binding Site for expression of…PromoterT7Available SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only