We narrowed to 30,836 results for: Ide

1. actc1b-mKate2-CaaX(tK)

   Plasmid

   #109488

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Kras

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   actc1b-mKate2-CaaX(tK)
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b T7pCONS sfGFP

   Plasmid

   #154467

   Purpose

   pET15b with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Tags

   His-thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Fast-GCaMP-EF20

   Plasmid

   #52645

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-EF20
   

   Expression

   Mammalian

   Mutation

   D362N/D366N mutated from GCaMP3

   Promoter

   T7

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. vas-Cas9.874Z

   Plasmid

   #112687

   Purpose

   Express hSpCas9 under vasa promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   vasa

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPM1001

   Plasmid

   #167548

   Purpose

   Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

   Depositor

   Renato Morona

   Article

   Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

   Insert

   Vibrio cholerae O antigens genes of the Inaba serotype
   

   Use

   Cosmid

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMT-PT2A-iRFP670-Tdtomato-GFP

   Plasmid

   #111817

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   iRFP670
   Tdtomato
   GFP
   

   Use

   Cloning intermediate

   Available Since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-wtSpCas9-H840A

   Plasmid

   #80453

   Purpose

   SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   SpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFP.R265

   Plasmid

   #138167

   Purpose

   Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(VDA).M86.(SDL)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-DUET-1-mRhubarb720-HO

   Plasmid

   #141201

   Purpose

   Expresses mRhubarb720 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb720
   heme oxygenase
   

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, L196Q, T202D, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEE393

    Plasmid

    #149283

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

11. pHH0103_RBM17_RRM

    Plasmid

    #109786

    Purpose

    Protein expression and purification of RBM17_RRM

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RBM17_RRM (RBM17 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCmini-UGCG-tdTomato

    Plasmid

    #225879

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by tdTomato insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    tdTomato
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV4.50-mut

    Plasmid

    #201059

    Purpose

    Expresses AAV2 Rep and AAV4.50 Cap

    Depositor

    Aravind Asokan

    Article

    Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

    Insert

    AAV4.50 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    N586R, S587A, N588L, L589R

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-NEAT1m_v1

    Plasmid

    #97083

    Purpose

    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1m_v1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJB158

    Plasmid

    #202601

    Purpose

    RP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-mFzd9

    Plasmid

    #42261

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 9 (Fzd9 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pAAV.Syn-FLEX.iGABASnFR.mRuby3

    Plasmid

    #112169

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR
    

    Use

    AAV

    Tags

    mRuby3

    Promoter

    Synapsin

    Available Since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4a-SIRT6-G60A-FLAG

    Plasmid

    #102323

    Purpose

    Human SIRT6 G60A with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glycine 60 to alanine

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TOPO Cre-2A-GFP

    Plasmid

    #68450

    Purpose

    TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Cre-2A-GFP
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-6p-1 Sesn2

    Plasmid

    #61873

    Purpose

    bacterial expression of a GST-Sesn2 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn2 (Sesn2 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only