We narrowed to 6,508 results for: 424

1. RVD-TT9

   Plasmid

   #173424

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Insert

   NG-NG dimer RVD at Position9
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   no

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. LLP672_L2_pV01_0I_VloxP-OCS-Rluc_lacZ

   Plasmid

   #192424

   Purpose

   To act as the off state for testing of the alt VCre recombinase.

   Depositor

   Ryan Lister

   Article

   Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

   Insert

   Act2::VloxP-OCS-VloxP::Rluc
   

   Use

   Synthetic Biology

   Tags

   PEST

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTL363

   Plasmid

   #168424

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   OsaHIP10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM328

   Plasmid

   #134243

   Purpose

   Mammalian expression construct expressing sgRNA against PLK1

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   gRNA targeting PLK1 3'
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. S3_007_026_MV2

   Plasmid

   #134424

   Purpose

   pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. nucHCB1

   Plasmid

   #97424

   Purpose

   Nuclear Huntingtin Chromobody 1: "Happ1" intrabody recognizing amino acids 41–81 of huntingtin, fused to eYFP and nuclear localization signal.

   Depositor

   Ray Truant

   Article

   Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.

   Insert

   nucHCB1
   

   Use

   Tags

   NLS and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

   Plasmid

   #84246

   Purpose

   Expresses direct fusion VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   VPR-tagBFP-Sa dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA-humanized

   Plasmid

   #44248

   Purpose

   Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid

    #84247

    Purpose

    Expresses direct fusion VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDOE11 Lat52:mCherry transient

    Plasmid

    #124249

    Purpose

    Allows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.

    Depositor

    Attila Fehér

    Article

    Lajko et al Plant Cell Rep. 2018 Apr;37(4):627-639.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT/TO-Flag-RNF169

    Plasmid

    #74243

    Purpose

    Expresses RNF169 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)

    Plasmid

    #86424

    Purpose

    PCR tagging module with yeast codon optimized mTurquoise2

    Depositor

    Sue Jaspersen

    Article

    Burns et al Elife. 2015 Sep 15;4. doi: 10.7554/eLife

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-ATG8(414)/GFP-AUT7(414)

    Plasmid

    #49424

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

    Depositor

    Daniel Klionsky

    Article

    Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pU6_(GLuc)_INT[3xPP7_SL]

    Plasmid

    #68424

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. RhoC FLARE.sc mCer, mVenus - CA

    Plasmid

    #65424

    Purpose

    RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, Q63L in RhoC

    Promoter

    Available since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTRE2-Bla(HA–RILP-FLAG)

    Plasmid

    #102424

    Purpose

    Dual tagged RILP for expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-Responsive

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKC158

    Plasmid

    #134242

    Purpose

    CENP-A Halo expression construct

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    CENP-A-Halo tagging construct (CENPA Human)
    

    Use

    Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. N118 nLV EF-1a-Gal-FKBPx1-HA-PGK-Blast

    Plasmid

    #44245

    Depositor

    Jerry Crabtree

    Article

    Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

    Insert

    FKBP (FKBP1A Human)
    

    Use

    Lentiviral

    Tags

    Gal4/DBD/aa1-147 and HA

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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