Addgene

We narrowed to 1,653 results for: NOI

Showing: 861 - 880 of 1653 results

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pDUET-ctCPR-A13AMO

Plasmid

#20114

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and amorphadiene oxidase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pLLU2G-Oct3

Plasmid

#21616

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pLLNeo-Oct3

Plasmid

#21618

Depositor

David Garbers

Article

Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Insert

Oct3 shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2-hcar1-4-mKate2

Plasmid

#226712

Purpose

overexpression red fluorescent hcar1-4 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

hcar1-4

Use

Unspecified; Zebrafish expression

Tags

mKate2

Expression

Mutation

Promoter

SP6

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP10

Plasmid

#212843

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP13

Plasmid

#212846

Purpose

Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD20

Plasmid

#208271

Purpose

Production of 13R-manoyl oxide in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
abCAS
cfTPS3

Use

Tags

Expression

Bacterial

Mutation

abCAS(D537A)

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJK392

Plasmid

#70236

Purpose

Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE-H40N

Depositor

T. J. Kappock

Article

Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

Insert

phosphoribosylaminoimidazole carboxylase

Use

Tags

Expression

Bacterial

Mutation

His40Asn

Promoter

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTagBFP2-blast

Plasmid

#125239

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTagBFP2 (codon optimized)

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-P2A-GFP-PGK-Puro

Plasmid

#110869

Purpose

Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-mNeonGreen. PGK-puro

Plasmid

#136255

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-GFP-PGK-Puro

Plasmid

#110866

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAP3K2

Plasmid

#85652

Purpose

retroviral expression of human MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

MAP3K2 (MAP3K2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-xFNLS-P2A-Puro

Plasmid

#110872

Purpose

Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xFNLS(3.7)

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-xBE4Gam-P2A-Puro

Plasmid

#112676

Purpose

Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE4Gam

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-xBE3-P2A-Puro

Plasmid

#110870

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)-BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-AR C619Y

Plasmid

#86429

Purpose

Fluorescent human androgen receptor (fused to EGFP) with point mutation C619Y

Depositor

Michael Mancini

Article

Nazareth et al Mol Endocrinol. 1999 Dec;13(12):2065-75.

Insert

Androgen receptor (AR) (AR Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C619Y

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG-FGFRdn

Plasmid

#80431

Purpose

Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

Depositor

Aixa Morales

Article

Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

Insert

Fibroblast growth factor receptor 1 (FGFR1 Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains aa 1–425

Promoter

beta-actin promoter

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only