Skip to main content
Addgene
Showing: 861 - 880 of 44034 results

  1. pX330-Flag-HypaSpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126756
    Purpose
    Expression plasmid for human codon-optimized increased fidelity HypaSpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126756-plasmid-map-sequence-id-410579

    Enlarge

  10. VV017: Archaerhodopsin-3 w/ D95Q point mutation fused to eGFP

    Plasmid
    #58490
    Purpose
    Expresses Arch(D95Q)-eGFP in mammalian cells as a fluorescent reporter of membrane voltage
    Depositor
    Adam Cohen
    Article
    Venkatachalam et al J Am Chem Soc. 2014 Feb 12;136(6):2529-3
    Insert
    Archaerhodopsin-3 w/ D95H point mutation fused to eGFP
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Changed Aspartic Acid 95 to Glutamine
    Promoter
    Ubiquitin
    Available since
    Aug. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    58490-plasmid-map-sequence-id-90354

    Enlarge

  11. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid
    #140227
    Purpose
    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    HRE (Hypoxia inducible)
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140227-plasmid-map-sequence-id-291996

    Enlarge

  12. WNV strain-NY99 NS2B-NS3 protease fusion with non cleavable linker with mutation K to A to reduce autoproteolysis

    Plasmid
    #228660
    Purpose
    Bacterial expression of the fusion of the NS2B-NS3 protease from West Nile virus with an H163A - catalytically inactive mutation for xxxxx
    Depositor
    Frank von Delft, ASAP Discovery
    Insert
    West Nile portion of NS2B protease fused to NS3
    Use
    Tags
    His-Twin Strep-Tev
    Expression
    Bacterial
    Mutation
    H163A - catalytically inactive/West Nile Virus NS…
    Promoter
    Available since
    Feb. 18, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    228660-plasmid-map-sequence-id-461601

    Enlarge

  13. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

    Plasmid
    #212498
    Purpose
    Encodes SARS-CoV-2 spike Q493R, Q498R, N501Y, Y505H for pseudovirus production
    Depositor
    Alejandro Balazs
    Article
    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0
    Insert
    SARS-CoV-2 Spike Q493R, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Q493R, Q498R, N501Y, Y505H
    Promoter
    CAG
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    212498-plasmid-map-sequence-id-432816

    Enlarge

  17. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

    Enlarge

Showing: 861 - 880 of 44034 results