We narrowed to 28,626 results for: tes

1. actc1b-mKate2-rab26

   Plasmid

   #109616

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to rab26

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab26 (rab26 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-EHD1a

   Plasmid

   #109564

   Purpose

   Multisite gateway vector for 3' tagging with EHD1a

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EHD1a (zgc:77049 Zebrafish)
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-EHD2a

   Plasmid

   #109568

   Purpose

   Multisite gateway vector for 3' tagging with EHD2a

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EHD2a
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-CaaX(tK)

   Plasmid

   #109540

   Purpose

   Multisite gateway vector for 3' tagging with the CaaX domain from Kras

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   CaaX domain from Kras
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-hygro-eGFP-STOP-HEC1

   Plasmid

   #114026

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-STOP-HEC1 (NDC80 Human)
   

   Expression

   Mammalian

   Mutation

   Start codon of Hec1 is mutated to stop codon

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSM13

   Plasmid

   #101644

   Purpose

   Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

   Depositor

   Thomas Hughes

   Article

   Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

   Insert

   meleCFP
   

   Tags

   6XHis

   Expression

   Bacterial

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCKtheoRaj12

   Plasmid

   #69942

   Purpose

   pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR

   Depositor

   Alfonso Jaramillo

   Article

   Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   TheoHHAzRaj12,cisRaj12,sfGFP
   

   Use

   Synthetic Biology

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCKtheoRaj11

   Plasmid

   #69939

   Purpose

   pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

   Depositor

   Alfonso Jaramillo

   Article

   Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   TheoHHAzRaj11,cisRaj11,sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCKbreak12

   Plasmid

   #69938

   Purpose

   pSTC2,cisRaj12-sfGFP, KanR

   Depositor

   Alfonso Jaramillo

   Article

   Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   CisRaj12-sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCKtheoRaj12-o11

    Plasmid

    #69941

    Purpose

    pSTC2,theoHHAzRaj12,cisRaj11-sfGFP, KanR

    Depositor

    Alfonso Jaramillo

    Article

    Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    TheoHHAzRaj12,cisRaj11,sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7opt in pCAGGS

    Plasmid

    #65974

    Purpose

    Human codon-optimized T7 polymerase

    Depositor

    Benhur Lee

    Article

    Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

    Insert

    T7-opt
    

    Expression

    Mammalian

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    elavl3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-dCas9-VP64

    Plasmid

    #47107

    Purpose

    Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    Flag, HA, SV40 NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag‐mTim3

    Plasmid

    #49208

    Purpose

    expresses Flag tagged mouse Tim3

    Depositor

    Lawrence Kane

    Article

    Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

    Insert

    Tim3 (Havcr2 Mouse)
    

    Tags

    FLAG and signal sequence

    Expression

    Mammalian

    Mutation

    no Tim3 signal sequence

    Promoter

    EF1a

    Available Since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. huCaV2.2 (pSAD442-1)

    Plasmid

    #62574

    Purpose

    Human CaV2.2 expression in mammalian cells

    Depositor

    Diane Lipscombe

    Article

    Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

    Insert

    CACNA1B (CACNA1B Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINK-TRABID (OTU+AnkUBD, aa 245-697)

    Plasmid

    #61590

    Purpose

    Expresses human TRABID (OTU+AnkUBD domains) in E. coli.

    Depositor

    David Komander

    Article

    Licchesi et al Nat Struct Mol Biol. 2011 Dec 11;19(1):6

    Insert

    TRABID (ZRANB1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-244 and aa 698-708.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINK-OTUD3 (OTU, aa 52-209)

    Plasmid

    #61412

    Purpose

    Expresses human OTUD3 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 210-398.

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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