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pDONR221-SLCO1C1_STOP

Plasmid

#161390

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLCO1C1 (SLCO1C1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC43A3_STOP

Plasmid

#161368

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC43A3 (SLC43A3 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

Plasmid

#169181

Purpose

Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)

Tags

No purification tag and nsp7-nsp8 fusion protein …

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-2

Plasmid

#162549

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-1

Plasmid

#162548

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-3

Plasmid

#162550

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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px330-Apob-4

Plasmid

#162551

Purpose

sgRNA targeting Apob

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

sgRNA targeting Apob (Apob Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CLV1 L2_pECIA2

Plasmid

#115100

Purpose

Bait vector CLV1 L2_pECIA2 should be used with prey vector CLV1 L2_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G75820 (CLV1 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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GSO2/EDA23 X079_pECIA2

Plasmid

#115111

Purpose

Bait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G44700 (GSO2 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK1 A6_pECIA2

Plasmid

#114967

Purpose

Bait vector SERK1 A6_pECIA2 should be used with prey vector SERK1 A6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G71830 (SERK1 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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CLV1 L2_pECIA14

Plasmid

#114900

Purpose

Prey vector CLV1 L2_pECIA14 should be used with bait vector CLV1 L2_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G75820 (CLV1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK3/BAK1 BAKNcFus_pECIA14

Plasmid

#114775

Purpose

Prey vector SERK3/BAK1 BAKNcFus_pECIA14 should be used with bait vector SERK3/BAK1 BAKNcFus_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G33430 (BAK1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK1 A6_pECIA14

Plasmid

#114767

Purpose

Prey vector SERK1 A6_pECIA14 should be used with bait vector SERK1 A6_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G71830 (SERK1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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MK01_HUMAN_D0

Plasmid

#79713

Purpose

This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK01 (MAPK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75242

Purpose

CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Rb379-928/C706F-pGex2TK

Plasmid

#21750

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928 of wt Rb …

Available Since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-mEtfdh-AAA6

Plasmid

#232317

Purpose

Expression of mouse mutant Etfdh (AAA6)

Depositor

Ivan Topisirovic

Article

Papadopoli et al bioRxiv 2024.10.25.620155

Insert

Etfdh (Etfdh Mouse)

Use

Lentiviral

Mutation

PAM Sequence Mutations: (G36C; T39C, C42T; C72T, …

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-mEtfdh-AAG6

Plasmid

#232315

Purpose

Expression of mouse mutant Etfdh (AAG6)

Depositor

Ivan Topisirovic

Article

Papadopoli et al bioRxiv 2024.10.25.620155

Insert

Etfdh (Etfdh Mouse)

Use

Lentiviral

Mutation

PAM Sequence Mutations: (G36C, T39C, C42T, C72T, …

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

Plasmid

#240228

Purpose

Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

Plasmid

#240229

Purpose

Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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