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173,176 results

Showing: 4401 - 4410 of 173176 results

  2. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  7. PGKp-GFP-YAP (5SA)

    Plasmid
    #174174
    Purpose
    Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.
    Depositor
    Rod Bremner
    Article
    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610
    Insert
    YAP (YAP1 Human)
    Use
    Lentiviral
    Tags
    3x FLAG tag
    Expression
    Mammalian
    Mutation
    Five LATS phosphorylation sites (S61, S109, S127,…
    Promoter
    PGK
    Available Since
    Aug. 19, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    174174-plasmid-map-sequence-id-340277

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  9. pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

    Viral Prep
    #137134-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 4401 - 4410 of 173176 results