21,196 results
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Plasmid#119749PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.DepositorTypeEmpty backboneTagsGSTExpressionBacterialPromotertac promoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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pJOE7706.1
Plasmid#135075PurposeA shuttle vector for heterologous HA production with Corynebacterium glutamicumDepositorTypeEmpty backboneExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
ExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…Available SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianPromoterCBh and U6Available SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-BSD
Plasmid#174821PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-BSD markerDepositorInsertPEmax-P2A-BSD
TagsP2A-BSD, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailable SinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF1A
Plasmid#172206PurposeExpresses KIF1A tagged with eGFP in mammalian cellsDepositorAvailable SinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-E-cadherin-RFP
Plasmid#79603PurposepHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging systemDepositorInsertCDH1 promoter region (CDH1 Human)
UseLentiviralAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
hAAVS1 1L TALEN
Plasmid#35431PurposeTALEN designed to target the human AAVS1 locusDepositorInserthAAVS1 1L TALEN
ExpressionMammalianAvailable SinceMay 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
ExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable SinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only