We narrowed to 26,944 results for: She

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pcDNA3-FLAG-MTOR-V2006I

Plasmid

#69012

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

V2006I

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

Plasmid

#107313

Purpose

Encoding aCamkII-mCherry-Cre

Depositor

Hui Yang

Article

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

Use

AAV

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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UPRT-mCh-Nluc-P2A-neo-UPRT

Plasmid

#135015

Purpose

Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

mCh-Nluc-P2A-neo
UPRT 5'UTR
UPRT 3'UTR

Use

Cryptosporidium expression

Tags

mCherry and Nluc-neo

Promoter

Cryptosporidium actin and enolase

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-OGDH_wobble

Plasmid

#110938

Purpose

OGDH ORF containing silent wobble mutations insensitive to corresponding shRNAs

Depositor

William Hahn

Article

Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

Insert

OGDH wobble cDNA (OGDH Human)

Use

Lentiviral

Mutation

15 silent wobble mutations corresponding to three…

Promoter

SV40

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-C1483F

Plasmid

#69008

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-C1483F (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Cysteine 1483 to Phenylalanine

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-syn-FLEX-jYCaMP1s

Plasmid

#135420

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Has Service

AAV1

Insert

jYCaMP1s

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Gata6

Plasmid

#128827

Purpose

dox-dependent expression of mouse Gata6

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Gata6 (Gata6 Mouse)

Use

Lentiviral

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Klf5

Plasmid

#128830

Purpose

dox-dependent expression of mouse Klf5

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Klf5 (Klf5 Mouse)

Use

Lentiviral

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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mCardinal-Keratin-17

Plasmid

#56164

Purpose

Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Keratin (KRT18 Human)

Tags

mCardinal

Expression

Mammalian

Mutation

V282L

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-wtGATA6-3XAU1 neo

Plasmid

#72618

Purpose

Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Lentiviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Promoter

TRE Tight

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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TCF4-DN in pLX303

Plasmid

#42592

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

31 first amino acids of hTcf4 have been deleted

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3.1 KSR2

Plasmid

#25968

Depositor

Rob Lewis

Article

Costanzo-Garvey et al Cell Metab. 2009 Nov . 10(5):366-78.

Insert

Kinase suppressor of Ras 2 (Ksr2 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pTRIPZ Myc2-mouse TTP aa S52 S178 MUT

Plasmid

#107001

Purpose

Expresses epitope-tagged double-mutant murine TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Mutation

Changed Ser52 to Ala and Ser178 to Ala

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pRetroX SgK269 WT

Plasmid

#68028

Purpose

expression of Sgk269 in mammalian cells

Depositor

Roger Daly

Article

Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

Insert

PEAK1 (PEAK1 Human)

Use

Retroviral

Tags

FLAG/myc, HA, and IRES-DsRed

Expression

Mammalian

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pYLCRISPR/Cas9pUbi-N

Plasmid

#106331

Purpose

Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Use

CRISPR

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

ZmUbi

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe 3XFLAG-wtGATA6-3XAU1 puro

Plasmid

#72607

Purpose

Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Retroviral

Tags

3XAU1 and 3XFLAG

Expression

Mammalian

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pL452-Sf3b1-K700E

Plasmid

#90425

Purpose

selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)

Depositor

Alex Minella

Article

Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Sf3b1 - left and right homology arms (Sf3b1 Mouse)

Use

Homology-directed repair vector

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Entry vector for gateway cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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