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PKC beta I DN

Plasmid

#16381

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Mutation

Dominant negative (kinase inactive): K371R

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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EZ-L175

Plasmid

#173025

Purpose

Encodes OptoAMP1 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p7270 MSCV-N-HA-IRES-PURO Empty

Plasmid

#163302

Purpose

Empty vector control

Depositor

Peter Howley, Elizabeth White

Article

White et al J Virol. 2014 Aug;88(15):8201-12. doi: 1

Type

Empty backbone

Use

Retroviral

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKLxR5

Plasmid

#149465

Purpose

Broad-Host range expression vector, RK2 and pUC origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCS4083

Plasmid

#131739

Purpose

Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRXTN

Plasmid

#47916

Purpose

pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.

Depositor

James Iglehart

Article

Kochupurakkal et al PLoS One. 2013 Jul 4;8(7):e68509. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

Tet

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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GA_HBV_MS2_(AmpR)

Plasmid

#179155

Purpose

Encodes the X, C and S genes of Hepatitis B Virus

Depositor

Paul Freemont

Article

Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

Insert

XCS Gene

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pFCiR5

Plasmid

#149479

Purpose

Broad-Host range expression vector, RSF1010 origin, CinR(AM) activator, PCin promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFSy(1.1)GW

Plasmid

#27232

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pRS-shTGFbeta1

Plasmid

#14973

Depositor

Joan Massague

Article

Thomas et al Cancer Cell. 2005 Nov . 8(5):369-80.

Insert

TGFbeta1 shRNA (Tgfb1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway destination vector

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pESC-ADE (V5 and HA)

Plasmid

#222489

Purpose

Co-expression of two genes in yeast with V5 and HA tags and ADE2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

HA and V5

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PKC gamma DN

Plasmid

#21239

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Mutation

K380R

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLEXY (pDN137)

Plasmid

#72656

Purpose

golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Type

Empty backbone

Use

Golden gate entry vector

Tags

mCherry-LEXY

Expression

Mammalian

Promoter

CMV-F

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pWLxR5

Plasmid

#149490

Purpose

Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBLxVi5

Plasmid

#167516

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, Violacein, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-Violacein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAB152

Plasmid

#101663

Purpose

Opto-T7RNAP*(563-F2)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PKC gamma WT

Plasmid

#21236

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
CMVTO-p450-FKBP-nTEVP

Plasmid

#179675

Purpose

ER-associated FKBP split nTEVP (Rapalog inducible)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

FKBP-nTEVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP2

Plasmid

#80658

Purpose

Encodes the synthetic coiled coil peptide SYNZIP2

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP2

Tags

6xHis

Expression

Bacterial

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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