We narrowed to 7,050 results for: plasmid dna
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Plasmid#183077PurposePlasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.DepositorInsertCas12c tracrRNA and crRNA ending with a HDV ribozyme
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH026_proD_Cas12c-multiplexing_CAM
Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRExpressionBacterialAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3
Plasmid#171588PurposeLentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoterDepositorInsertsBxb1 integrase
iCasp9
BFP
rtTA3G
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCA11-pMa-sgTS3-17xMS2V5-sgTS3
Plasmid#199442PurposeCRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology armsDepositorInsertHITI donor template
UseCRISPRAvailable SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ202
Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorExpressionPlantPromoterArabidopsis ubiquitin 10 (AtUbi10)Available SinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pP2A_NLS_sfGFP
Plasmid#63709PurposeExpressing P2A_NLS_sfGFP with homologous arm of Nanog.DepositorInsertNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
UseJust a donor dna with nanog homologous arm for kn…TagssfGFPAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LLP783_SpyCatcher-DNMT3A-CO
Plasmid#211776PurposeSpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertTraptavudin-DNMT3A
Tags3xV5ExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP784_SnoopCatcher-DNMT3A-CO
Plasmid#211777PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertSpy-EZH2
Tags3xFLAGExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect EZH2 e…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP789_αGCN4-DNMT3A-CO
Plasmid#211783PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertaGCN4-DNMT3A
Tags3xTy1ExpressionMammalianMutationLast 300 bp codon optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRpr150-LUC
Plasmid#85700Purposeluciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)DepositorInsertRpR 150 enhancer and hsp70 minimal promoter
UseLuciferaseTagsluciferaseExpressionMammalianPromoterRpR 150 enhancer and hsp70 minimal promoterAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only