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pAAV-hSyn-DIO {ChETA}on-WPRE

Plasmid

#111386

Purpose

AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Insert

ChETA

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

humanized ChR2, E123T/H134R

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-pShuttle

Plasmid

#122122

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Tags

Omni

Expression

Mammalian

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p)

Plasmid

#70211

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

deCFP

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAJT430

Plasmid

#202291

Purpose

RPU integration plasmid (LPattB7)

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

J23101-RiboJ00-B0034-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCLC-mEos2

Plasmid

#38010

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

mEos2

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pMaCTag-P24

Plasmid

#120035

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P18

Plasmid

#120029

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-06

Plasmid

#119985

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with sfGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

sfGFP

Use

Pcr template

Tags

sfGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAJT254-litR

Plasmid

#202308

Purpose

litR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

litR

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT249-psrA

Plasmid

#202304

Purpose

psrA repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

psrA

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Article

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P10

Plasmid

#120021

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hEFA6B

Plasmid

#206413

Purpose

Mammalian expression of hEFA6B

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6B (PSD4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z33

Plasmid

#120050

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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LentiU6-hNEUROD1 gRNA_1-MS2-Puro

Plasmid

#192676

Purpose

Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human NEUROD1 activating gRNA #1 (NEUROD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoAmax-BlastR

Plasmid

#152996

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

evoA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 AT1R DRY-AAY

Plasmid

#45759

Depositor

Laszlo Hunyady, Robert Lefkowitz

Article

Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

Insert

AT1R DRY-AAY (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

changed amino acids 125-126 from DR to AA

Promoter

CMV

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pRSM17

Plasmid

#101641

Purpose

Encodes the fluorescent protein eqFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

eqFP486

Tags

6XHis

Expression

Bacterial

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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