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MTK3_036

Plasmid

#123750

Purpose

Encodes mScarlet as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-MCH

Plasmid

#184606

Purpose

For expression of a fluorescent sensor for melanin-concentrating hormone in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-MCH

Expression

Mammalian

Promoter

CAG promoter

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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dGFP-Rabphilin

Plasmid

#170866

Purpose

Mammalian expression of dGFP-Rabphilin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rabphilin-3a (RPH3A Bovine)

Tags

dEGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJPT003-PM-HaloTag-1x FLAG

Plasmid

#202494

Purpose

Express "PM" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Tags

1x FLAG and Palmitoylation-Myristoylation Site

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV-E

Plasmid

#191555

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.E

Plasmid

#191553

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y925F

Plasmid

#50509

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y925F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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HI.fate.UB

Plasmid

#191556

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV

Plasmid

#191554

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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YH001 CMV-TO-tm-GS+XTEN-ADAR2(DD-E396A)-RXR (FLP-IN)

Plasmid

#233383

Purpose

ER-retained catalytically inactive ADAR2dd (E396A mutation)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

TM-ADAR2dd

Expression

Mammalian

Mutation

E396A

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

Plasmid

#159912

Purpose

Mutagenesis of Kcnd3

Depositor

Larry Zweifel

Insert

Kcnd3 (Kcnd3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

Plasmid

#13005

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 1 delta CC (SULF1 Human)

Expression

Mammalian

Mutation

changed Cysteine 87 to Alanine

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only
LV anti-FITC-LaG16-Y186F-T2A-BFP

Plasmid

#194931

Purpose

LV construct encoding Y186F mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA_RFP-SZ2

Plasmid

#124936

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

mCherry

Expression

Mammalian

Mutation

SZ2 domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-NbALFA

Plasmid

#207627

Purpose

Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-NbALFA

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS1938

Plasmid

#82563

Purpose

High copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Baumann et al Exp Eye Res. 2019 Jul 16:107728. doi: 10

Insert

Ple261

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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hBax S184E C3-EGFP

Plasmid

#19743

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax S184E (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 184 to Glutamate

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only