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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,931 results for: cas9

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Showing: 8941 - 8960 of 25931 results

  2. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid
    #140199
    Purpose
    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Promoter
    PgpdA
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140199-plasmid-map-sequence-id-309723

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  3. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid
    #121833
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    x-dCas9(3.7)/Dnmt3a-3L (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121833-plasmid-map-sequence-id-237387

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  4. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid
    #198551
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8.17-m+V106W
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198551-plasmid-map-sequence-id-388896

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  5. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

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  7. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid
    #202009
    Purpose
    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP
    Depositor
    Michael Smanski
    Publication
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-24XGP41
    Use
    Synthetic Biology; Moclo level 1 vector
    Tags
    GS linkers
    Promoter
    CmYLCV
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    202009-plasmid-map-sequence-id-401182

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  8. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid
    #183876
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ANLN sgRNA spacer (ANLN Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    183876-plasmid-map-sequence-id-390709

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  9. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

    Plasmid
    #148859
    Purpose
    Mammalian Expression of HsXRN1-sgRNA
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsXRN1-sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148859-plasmid-map-sequence-id-478935

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  10. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

    Plasmid
    #148855
    Purpose
    Mammalian Expression of HsAGO3-sgRNA
    Depositor
    Elisa Izaurralde
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HsAGO3-sgRNA (AGO3 S.pyogenes)
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148855-plasmid-map-sequence-id-401013

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  13. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid
    #183884
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTB sgRNA spacer (ACTB Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    183884-plasmid-map-sequence-id-362436

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  14. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid
    #183888
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TUBA1B sgRNA spacer (TUBA1B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183888-plasmid-map-sequence-id-362425

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  15. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid
    #183877
    Purpose
    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RHOA sgRNA spacer (RHOA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183877-plasmid-map-sequence-id-362410

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Showing: 8941 - 8960 of 25931 results