We narrowed to 69,664 results for: kan

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YFP-Cdc42(N17)

Plasmid

#11400

Depositor

Joel Swanson

Publication

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cell division cycle 42 (CDC42 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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YFP-Cdc42(N17)

Plasmid

#11400

Depositor

Joel Swanson

Publication

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cell division cycle 42 (CDC42 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Available since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SPYtag-GFPnanobody

Plasmid

#214837

Purpose

This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Publication

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-GFPnanobody

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Publication

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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ATG9A-mCherry

Plasmid

#197393

Purpose

Expresses ATG9A in mammalian cells

Depositor

Juan Bonifacino

Publication

De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATG9A (ATG9A Human)

Tags

mCh

Expression

Mammalian

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-His6

Plasmid

#182386

Purpose

Bacterial expression of Vesicle Nucleating peptide

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-ROCK1

Plasmid

#187296

Purpose

Express GFP ROCK1

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Publication

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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LBR-mCherry

Plasmid

#172446

Purpose

Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

Depositor

Stephen Royle

Publication

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

lamin B receptor (LBR Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Publication

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Publication

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Publication

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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plRL117

Plasmid

#163635

Purpose

Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Orf3a-mCherry

Plasmid

#165138

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Publication

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Orf3a (ORF3a codon optimized SARS-COV-2)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJW2098

Plasmid

#163094

Purpose

CRISPR repair template: add insertion site specific homology arms by PCR before insertion

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker

Use

CRISPR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPP381

Plasmid

#158798

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-2

Tags

6xHis

Expression

Bacterial

Mutation

RuvC-inactivating mutation D394A

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Publication

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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LBR EGFP

Plasmid

#128150

Purpose

Expression of lamin B receptor

Depositor

Jennifer Lippincott-Schwartz

Publication

Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

lamin B receptor (LBR Human)

Expression

Mammalian

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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