We narrowed to 58,607 results for: plasmid
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Plasmid#60972PurposeExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).DepositorInsertddRFP A and ddRFP B
TagsNES sequence LALKLAGLDIGS and triplicated NLS seq…ExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti_Ef1a:PE2-P2A-GFP
Plasmid#184445PurposeSingle EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and Ef1AAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)
Plasmid#122985PurposeAAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNTDepositorInsertmCh-IRES-SspB(milli)-BoNT/B(147-441)
UseAAVTagsmChExpressionMammalianMutationSspB contains A58V,R73Q "milli" mutatio…Promoterhuman synapsinAvailable SinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS1986
Plasmid#49117PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-iCre-WPRE
UseAAVExpressionMammalianPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable SinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-Tet1 promoter A
Plasmid#63881PurposeLuciferase reporter plasmid used to study transcriptional control of murine Tet1DepositorInsertTet1 promoter fragment A
UseLuciferaseExpressionMammalianAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)
Plasmid#125146PurposeAn adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoterDepositorInsertHA-tagged hM4D(Gi) (CHRM4 Human)
UseAAV and Cre/LoxTagsHAExpressionMammalianPromoterSYN1Available SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDHC29
Plasmid#80781PurposeCloning vector compatible with ColE1 plasmids with high copy numberDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRII-BbsI-sgRNAscaffold
Plasmid#159352PurposeExpression vector for a customizable guide RNADepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterU6Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IL-2 signal_rabbit Fc
Plasmid#128031PurposeMammalian expression vector with N terminal IL-2 signal peptide and rabbit FcDepositorTypeEmpty backboneTagsIL-2 signal peptide and rabbit FcExpressionMammalianAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.17_Ex_Ef1a-v5-EGFP-SV40-Zeo
Plasmid#128453Purposelentiviral expression vector with Ef1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and ZeoResistance geneDepositorInsertEf1a promoter, N-terminal V5 tag, EGFP gene, SV40 promoter, and Zeocin Resistance gene
UseLentiviralAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOP5MP
Plasmid#112612PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneTags8His-MBP and AviExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDS-Red-N1 GABAA beta3
Plasmid#109143PurposeThe expression vector carries the red fluorescent protein tagged at the C-terminus of beta3 subunit of the GABAa receptor.DepositorInsertGABAa receptor
TagsRFPExpressionMammalianMutationbeta3 subunit of the GABAa receptorPromoterCMVAvailable SinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215010PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianAvailable SinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-iRFP670
Plasmid#133397PurposePiggybac vector for constitutive expression of iRFP670-NLSDepositorInsertiRFP-670-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN1-OPA1(123Nt)-Halo
Plasmid#129345PurposeHalo Tag, Mitochondrial intermembrane space localizationDepositorInsertHalo (Promega)
ExpressionMammalianAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Eya2
Plasmid#49265Purposeretroviral expression of Eya2 and YFPDepositorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
L5-LexAp65-L2
Plasmid#41437DepositorInsertLexAp65
ExpressionBacterialAvailable SinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCS2+N-3xHA Cloning Vector
Plasmid#196653PurposeN-terminal 3xHA mammalian expression plasmidDepositorTypeEmpty backboneTags3xHAExpressionMammalianPromoterCMVAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only