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(318) pcDNA3.1-myc-OGA(1-400)D174N

Plasmid

#168096

Purpose

Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

myc-OGA(1-400, D174N)

Tags

myc

Expression

Mammalian

Mutation

D174N catalytically inactive OGA

Promoter

T7/CMV

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD9-(TEV)-tTA

Plasmid

#162596

Purpose

human CD9-TEV cleavage site-tTA

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD9 (CD9 Human)

Tags

TEV protease cleavage site and tTA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

Plasmid

#132410

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dEsCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R295A; H300A; R849A; H854A

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pPN440

Plasmid

#137874

Purpose

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:PYL1:nls:VPR

Plasmid

#135988

Purpose

Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

PYL1 domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_AURKB

Plasmid

#111682

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AURKB (AURKB Human)

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NL-BCL2L1

Plasmid

#113458

Purpose

NanoLuc-BCL2L1 expression vector (positive control together with pcDNA3.1 PA-mCit-BAD)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 like 1 (BCL2L1 Human)

Use

Luciferase

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-4eCOL2A1

Plasmid

#97211

Purpose

For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

Depositor

Ryan Porter

Article

De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

Insert

4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)

Use

Vector is designed for cloning and generation of …

Promoter

COL2A1 regulatory elements

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PU.1-ets-IRES-mCherry

Plasmid

#80141

Purpose

retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Mutation

ets = PU.1 DNA binding domain (amino acids 159-26…

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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Cav3P104L-YFP

Plasmid

#68404

Purpose

Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells

Depositor

Rob Parton

Article

Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105.

Insert

Cav3 (Cav3 Mouse)

Tags

EYFP

Expression

Mammalian

Mutation

Proline at position 104 changed to a Leucine

Promoter

CMV

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 AT1R DRY-AAY

Plasmid

#45759

Depositor

Laszlo Hunyady, Robert Lefkowitz

Article

Gaborik et al Endocrinology. 2003 Jun;144(6):2220-8.

Insert

AT1R DRY-AAY (Agtr1a Rat)

Tags

HA

Expression

Mammalian

Mutation

changed amino acids 125-126 from DR to AA

Promoter

CMV

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 Notch1 LNG-6MT

Plasmid

#41738

Depositor

Raphael Kopan, Jeffrey Nye

Article

Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

Insert

Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 leader peptide (aa 1-23) …

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B Synthetic, HDR template, Human)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DDR1-V5/HIS

Plasmid

#201100

Purpose

expression of human DDR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBK086

Plasmid

#183145

Purpose

pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-sfGFP*-XcP4H-MH4R

Plasmid

#178043

Purpose

Express green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTP

Depositor

Han Xiao

Article

Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

Inserts

superfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human

Tags

His tag

Expression

Bacterial

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-Munc18-1wt

Plasmid

#135550

Purpose

Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18-1wt

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PEX-mCherry-CRY2

Plasmid

#102250

Purpose

Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

PEX-mCherry-CRY2 (PEX3, CRY2 Mustard Weed, Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-mFgf21pro3-luc

Plasmid

#101799

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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