Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 12,110 results for: NSI

Clear keywords
Showing: 1761 - 1800 of 12110 results

  6. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

    Plasmid
    #153442
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    153442-plasmid-map-sequence-id-310821

    Enlarge

  15. pANAP-P2A-eRF(E55D)

    Plasmid
    #241290
    Purpose
    Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor
    Depositor
    Justin Taraska
    Article
    Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003
    Insert
    the amber suppressor tRNACUA-Leu
    Expression
    Mammalian
    Mutation
    mutant E.coli leucyl synthetase specific for Anap…
    Promoter
    CMV
    Available Since
    Dec. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241290-plasmid-map-sequence-id-481082

    Enlarge

  17. pCS2+8-CYP2A6(C14A, C439A)-flag-tev-halo-his

    Plasmid
    #227162
    Purpose
    For T-REX experiments of CYP2A6 C14A and C439A double mutant. Less sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 14 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227162-plasmid-map-sequence-id-459059

    Enlarge

  18. pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

    Plasmid
    #227158
    Purpose
    For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    cyp-33e1 (cyp-33E1 Nematode)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 295 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227158-plasmid-map-sequence-id-458998

    Enlarge

  20. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

    Plasmid
    #227163
    Purpose
    For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).
    Depositor
    Yimon Aye
    Article
    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.
    Insert
    CYP2A6 (CYP2A6 Human)
    Tags
    Flag, HaloTag, and His6
    Expression
    Mammalian
    Mutation
    changed cysteine 82 and cysteine 439 to alanine
    Promoter
    CMV and SP6
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    227163-plasmid-map-sequence-id-459000

    Enlarge

  28. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

    Plasmid
    #153445
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153445-plasmid-map-sequence-id-306484

    Enlarge

  29. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

    Plasmid
    #153444
    Purpose
    E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Tags
    6xHis and ybbr tag
    Expression
    Bacterial
    Mutation
    Serine 199 was mutated to amber codon (TAG). Argi…
    Promoter
    T7 promoter
    Available Since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153444-plasmid-map-sequence-id-306456

    Enlarge

  31. pcDNA4/TO-ORF66 C423A-2xStrep

    Plasmid
    #131111
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 423 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131111-plasmid-map-sequence-id-257476

    Enlarge

  32. pcDNA4/TO-ORF66 C399A-2xStrep

    Plasmid
    #131112
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 399 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131112-plasmid-map-sequence-id-257485

    Enlarge

  33. pcDNA4/TO-ORF66 C393A-2xStrep

    Plasmid
    #131113
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 393 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131113-plasmid-map-sequence-id-257490

    Enlarge

  34. pcDNA4/TO-ORF66 C355A-2xStrep

    Plasmid
    #131114
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 355 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131114-plasmid-map-sequence-id-257492

    Enlarge

  35. pcDNA4/TO-ORF66 C344A-2xStrep

    Plasmid
    #131115
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 344 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131115-plasmid-map-sequence-id-257501

    Enlarge

  36. pcDNA4/TO-ORF66 C341A-2xStrep

    Plasmid
    #131116
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 341 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131116-plasmid-map-sequence-id-257506

    Enlarge

  37. pcDNA4/TO-ORF66 C301A-2xStrep

    Plasmid
    #131117
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C301A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 301 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131117-plasmid-map-sequence-id-257511

    Enlarge

  38. pcDNA4/TO-ORF66 C298A-2xStrep

    Plasmid
    #131118
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C298A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 298 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131118-plasmid-map-sequence-id-257518

    Enlarge

  39. pcDNA4/TO-ORF66 C295A-2xStrep

    Plasmid
    #131119
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C295A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 295 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131119-plasmid-map-sequence-id-257521

    Enlarge

  40. pcDNA4/TO-ORF66 C289A-2xStrep

    Plasmid
    #131120
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C289A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 289 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131120-plasmid-map-sequence-id-257523

    Enlarge

Showing: 1761 - 1800 of 12110 results