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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,533 results for: Gaa

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Showing: 881 - 900 of 4533 results

  3. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210735
    Purpose
    Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Gollum sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210735-plasmid-map-sequence-id-420080

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  7. pSCL.111

    Plasmid
    #184980
    Purpose
    Test effect of extending a1/a2 on TRP2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    TRP2 donor E64stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184980-plasmid-map-sequence-id-376669

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  10. pPBO.ACT004

    Plasmid
    #182714
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182714-plasmid-map-sequence-id-364194

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  11. 1056H

    Plasmid
    #183135
    Purpose
    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.
    Depositor
    Omar Akbari
    Article
    Kandul et al GEN Biotechnology (2022) 1:4, 372-385
    Insert
    U6.3-gRNAs[sxl, bTub]
    Use
    CRISPR
    Expression
    Insect
    Available Since
    June 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    183135-plasmid-map-sequence-id-362097

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  15. pSLQ1371-sgYAP-10

    Plasmid
    #121424
    Purpose
    sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.
    Depositor
    Ovijit Chaudhuri, Stanley Qi
    Article
    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:
    Insert
    sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121424-plasmid-map-sequence-id-364370

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Showing: 881 - 900 of 4533 results