Addgene

We narrowed to 10,641 results for: cat.1

Showing: 881 - 900 of 10641 results

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pORANGE CACNG8-GFP KI #1

Plasmid

#131473

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - Amplicon, JAK2 sgRNA 1

Plasmid

#70679

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Feb 8. ():.

Insert

INO80 (INO80 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Promoter

Available since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP:HA-mTREK-1(S131C)

Plasmid

#224774

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

S131C

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem wt-S131C)

Plasmid

#224759

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem wt-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (wt-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S562C

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(tandem S131C-wt)

Plasmid

#224758

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-wt)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-wt) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-48-GFP

Plasmid

#108507

Purpose

expression of vsv-INCENP 1-48-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-58-GFP

Plasmid

#108508

Purpose

expression of vsv-INCENP 1-58-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-63-GFP

Plasmid

#108509

Purpose

expression of vsv-INCENP 1-63-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (1-249)

Plasmid

#42204

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-249) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-249

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
LentiCRISPR v2 RB1 sgRNA #1

Plasmid

#202516

Purpose

Expressing Cas9 and sgRNA targeting human RB1 gene

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

RB1 sgRNA (RB1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (1-397)

Plasmid

#42205

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (1-397) (SRC Human)

Use

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 1-397

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
NC1 pGLUE WTX (1-804)

Plasmid

#36956

Depositor

Randall Moon

Article

Major et al Science. 2007 May 18;316(5827):1043-6.

Insert

WTX (AMER1 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pGEMHE:mTREK-1(tandem S131C-S131C)

Plasmid

#224760

Purpose

In vitro transcription for Oocyte expression of mTREK-1(tandem S131C-S131C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Tandem TREK-1 (S131C-S131C) (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C / S562C

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131A/C159A/C219A)

Plasmid

#224753

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131A/C159A/C219A

Promoter

T7

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (S131C/C159A/C219A)

Plasmid

#224752

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Tags

Expression

Mutation

S131C/C159A/C219A

Promoter

T7

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mCct8 - 1

Plasmid

#198503

Purpose

lentiviral stable expression of mCct8 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mCct8 (Cct8 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1(nsp8-nsp7)(nsp12)

Plasmid

#165451

Purpose

Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

Depositor

Marc Delarue

Article

Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

Inserts

nsp12
nsp8
nsp7

Use

Tags

14Histidine-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE (hygro) hYAP 1-1delta

Plasmid

#124150

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Marius Sudol, Megan Finch-Edmondson

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Tags

Expression

Mutation

None (Wild Type)

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Repo-Man-1-890

Plasmid

#184272

Purpose

Expresses C-terminally truncated GFP-RepoMan

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

truncated Repo-Man after aminoacid 893

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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