We narrowed to 25,285 results for: crispr
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Plasmid#62654PurposeArabinose inducible lambda red and anhydrotetracycline inducible sgRNA expressionDepositorInsertexo, beta, gam, sgRNA
ExpressionBacterialAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRT043-CLYBL-DDdcas9-VPH
Plasmid#158091PurposeInducible expression of dCas9-VPH from the CLYBL locus for CRISPR activationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLYBL-CAG-DDdCas9VPH-T2A-GFP
UseTALENExpressionMammalianPromoterCAGAvailable sinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPaint Gene Tagging Kit
Plasmid kit#1000000086PurposeCRISPR-assisted insertion tagging (CRISPaint) plasmid collection for modular integration of large DNA fragments into user-defined genomic locations via a ligase-4-dependent mechanism.DepositorApplicationGenome EditingVector typeMammalian ExpressionEditing typeCRISPRAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCB578
Plasmid#141095PurposeE. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat arrayDepositorInsertsCas9 and tracrRNA
repeat-spacer-repeat array
ExpressionBacterialAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PE6b
Plasmid#207852PurposeMammalian expression of PE6b prime editorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE6b
TagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationTf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…Available sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RNA-binding protein pooled CRISPR knockout library
Pooled library#141438PurposeKnockout library targeting RNA-binding proteins with 10 guide RNAs per target.DepositorExpressionMammalianUseCRISPR and LentiviralAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-tet-iCas9-BFP2
Plasmid#125519Purposeall-in-one-inducible Cas9DepositorInsertAAVS1-TET-ON 3G-SpCas9-P2A-BFP2
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-FNLS-P2A-Puro
Plasmid#110841PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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Bassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Pooled library#101927PurposeBassik lab Human CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases. Coexpresses mCherry.DepositorExpressionMammalianUseCRISPRAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP
Plasmid#188777PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Human sgRNA library Brunello in lentiGuide-Puro
Pooled library#73178PurposeHuman sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controlsDepositorHas serviceLentiviral PrepExpressionMammalianUseCRISPR and LentiviralAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag-3xNES-ABE8e
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SaBE4-Gam
Plasmid#100809PurposeExpresses SaBE4-Gam in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSaBE4-Gam
ExpressionMammalianPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ABE8.20-m
Plasmid#136300Purposeexpresses ABE8.20-m in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8.20-m
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580AAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only