We narrowed to 5,962 results for: crispr cas9 expression plasmids

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pLL8 lenti-D10Acas9-3xflag-blast

Plasmid

#112134

Purpose

lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

D10Acas9-3xflag-blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A mutant in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pZac2.1 SV40-CMV-SaCas9-3xNLS

Plasmid

#78601

Purpose

AAV vector containing SaCas9

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

SV40-CMV-SaCas9-3xNLS

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

SV40, CMV promoters

Available Since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only
pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78606

Purpose

pZac2.1 CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

CMV173-SaCas9-U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

173CMV, U6

Available Since

Dec. 16, 2017

Availability

Academic Institutions and Nonprofits only
lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

Plasmid

#159086

Purpose

Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

Depositor

Jeremy Day

Article

Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

Insert

empty crRNA-acRNA backbone, dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

hSYN, U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-AAVS1_sgRNA

Plasmid

#183890

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+L1111R)-P2A-EGFP (LRF167)

Plasmid

#242673

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L1111R mutations in SpCas9(L1111R/D1135V/G…

Promoter

CMV and T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+A1322R)-P2A-EGFP (LRF59)

Plasmid

#242679

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + A1322R mutations in SpCas9(D1135V/G1218R/A…

Promoter

CMV and T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

Plasmid

#242666

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+N394K)-P2A-EGFP (KAC956)

Plasmid

#242670

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + N394K mutations in SpCas9(N394K/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+D269A)-P2A-EGFP (LRF64)

Plasmid

#242668

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + D269A mutations in SpCas9(D269A/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+G366R)-P2A-EGFP (KAC1026)

Plasmid

#242669

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + G366R mutations in SpCas9(G366R/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+N1286Q)-P2A-EGFP (LRF247)

Plasmid

#242677

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + N1286Q mutations in SpCas9(D1135V/G1218R/N…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

Plasmid

#242671

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

Plasmid

#242665

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

Plasmid

#242667

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

Plasmid

#242678

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

Plasmid

#242676

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

Plasmid

#242675

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

Plasmid

#242681

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

Plasmid

#242672

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…

Promoter

CMV and T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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