We narrowed to 2,003 results for: ipp

1. pPV-C1-crRNA-cloning-EF1a-BA

   Plasmid

   #204623

   Purpose

   piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)

   Plasmid

   #158771

   Purpose

   Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   Synaptotagmin 1 1-NS3/4a (Syt1 Mouse)
   

   Use

   Lentiviral

   Mutation

   Flexible linkers, ADVVCC/QMSY cleavage site

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO/MACROD1

   Plasmid

   #172578

   Purpose

   For generation of FlpIn lines with untagged MACROD1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD1 (MACROD1 Human)
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

   Plasmid

   #172583

   Purpose

   For transient expression of C-terminal BirA-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ8636 PB-PGK-RR12EE345L-C term (406) dLbCpf1-N intein-pA

   Plasmid

   #140223

   Purpose

   Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to intein for 3-way split activator

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to N-intein
   

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   PGK

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-myc-Sema4A RNAiR

   Plasmid

   #115172

   Purpose

   expresses RNAi-resistant Sema4A with N-terminal myc tag

   Depositor

   Suzanne Paradis

   Article

   McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

   Insert

   Sema4A (Sema4a Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Silent mutation between amino acids in ORF (401-4…

   Promoter

   CMV

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCML 313

   Plasmid

   #98586

   Purpose

   pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Insert

   csrB
   

   Expression

   Bacterial

   Promoter

   pLacO

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCML 314

   Plasmid

   #98584

   Purpose

   pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Inserts

   CsrA
   csrB
   

   Expression

   Bacterial

   Promoter

   pLacO

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. tdEos-Cx43-7

   Plasmid

   #57603

   Purpose

   Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Cx43
   

   Tags

   tdEos

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRSETC PITPbeta N89F

    Plasmid

    #58279

    Purpose

    bacterial expression of PITP beta N89F mutant

    Depositor

    Shamshad Cockcroft

    Article

    Carvou et al J Cell Sci. 2010 Apr 15;123(Pt 8):1262-7

    Insert

    PITP beta (PITPNB Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N89F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pFA6a-His3MX6-PGAL1-GST

    Plasmid

    #41613

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid

    #158768

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Mutation

    Flexible linkers between the protease and msGFP a…

    Availability

    Academic Institutions and Nonprofits only

    

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15. pF(UG) hSyn SYP-deltaTMD SYT7alpha

    Plasmid

    #179718

    Purpose

    Lentiviral expression of synaptic vesicle-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

    Plasmid

    #179719

    Purpose

    Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

    Plasmid

    #179722

    Purpose

    Lentiviral expression of lysosome-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179723

    Purpose

    Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pF(UG) U6-SYT7 sgRNA 777 HaloTag

    Plasmid

    #179724

    Purpose

    U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    sgRNA and HaloTag
    

    Use

    Lentiviral

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pF(UG) CamKII sf iGluSnFR S72A

    Plasmid

    #179726

    Purpose

    Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    iGluSnFR
    

    Use

    Lentiviral

    Mutation

    S72A

    Availability

    Academic Institutions and Nonprofits only

    

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