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pCDH-FLAG-R-Ras2

Plasmid

#102335

Purpose

Human R-Ras2 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Elife. 2017 Apr 13;6. pii: e25158. doi:

Insert

RRAS2 (RRAS2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (AAV5)

Viral Prep

#198516-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE (#198516). In addition to the viral particles, you will also receive purified pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of the optimized opsin PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

EF-1a

Tags

mScarlet

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only
PABPC1

Plasmid

#155805

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PABPC1 (PABPC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS7-egfp

Plasmid

#112917

Purpose

Fluorescence microscopy

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Insert

eGFP

Expression

Bacterial

Promoter

PS7 promoter and Ps7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSB-TRE-CRISPRoff-EF1A-TetOn

Plasmid

#203355

Purpose

Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

Depositor

Jonathan Brunger

Article

Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

Insert

CRISPRoff-TetOn

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-NLS

Plasmid

#199663

Purpose

expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

NLS and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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L304-EGFP-Tubulin-K40R

Plasmid

#64059

Purpose

EGFP fused at the N-terminus of tubulin with a mutation K40R; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pQFlag-USP11 WT puroR

Plasmid

#46747

Purpose

Retroviral vector that expresses Flag-tagged wild type human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV1.X1

Plasmid

#209780

Purpose

non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV1-X1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII

Plasmid

#45064

Purpose

Expression of CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Promoter

RSV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM60-Tiar

Plasmid

#38244

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tiar (Tial1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pCAGGS-PKCdelta-EGFP

Plasmid

#178856

Purpose

Mammalian expression of PKCδ fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ (PRKCD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-4HA

Plasmid

#18031

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4HA (Wnt4 Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-p18G2A-HA

Plasmid

#42327

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 G2A (LAMTOR1 Human)

Tags

HA

Expression

Mammalian

Mutation

G2A

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-OTI-TCRB_p2_TCRA

Plasmid

#131034

Purpose

Expresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRB (Tcrb Mouse)
TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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FGA-bio-His

Plasmid

#52029

Purpose

Expresses full-length Fibrinogen alpha chain precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

FGA (FGA Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40A

Plasmid

#105304

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Article

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40A

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-DYRK1A-2A-GFP

Plasmid

#118270

Purpose

Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)

Use

AAV

Tags

2A peptide and GFP

Expression

Mammalian

Promoter

CMV with beta global intron

Available Since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-FLAG-R-Ras2

Plasmid

#102335

Purpose

Human R-Ras2 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Elife. 2017 Apr 13;6. pii: e25158. doi:

Insert

RRAS2 (RRAS2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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