We narrowed to 42,102 results for: Eras

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pmCherry-3xNLS-OptoJNKi-dsm

Plasmid

#89743

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi dark-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

C450A mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-3xNLS-OptoJNKi-lsm

Plasmid

#89742

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi lit-state mutant

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Mutation

I539E mutation of AsLov2Jalpha

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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KC1E_HUMAN_D0

Plasmid

#79724

Purpose

This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1E (CSNK1E Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KRAS-V14I

Plasmid

#154427

Purpose

V14I Kras with Flag tag inserted into N-terminal domain between 6Xhis tag and TEV cleavage site.

Depositor

Kenneth Westover

Article

Bera et al J Biol Chem. 2019 Sep 20;294(38):13964-1

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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DAPK3_HUMAN_D0

Plasmid

#79721

Purpose

This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK3 (DAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Triple ORF FRT ifgMosaic Donor (IG135)

Plasmid

#99626

Purpose

To clone the 3 ORFs for FlpO dependent generation of ifgMosaics

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

PGK-Neo

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-Luc-KLF4-K1S

Plasmid

#62979

Purpose

Translational reporter for KLF4

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 partial sequence (KLF4 Human)

Use

Luciferase

Tags

firefly luciferase

Mutation

Fragment 1-446 of KLF4

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
EPHB3_HUMAN_D0

Plasmid

#79732

Purpose

This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB3 (EPHB3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHA3_HUMAN_D0

Plasmid

#79708

Purpose

This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHA3 (EPHA3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB2_HUMAN_D0

Plasmid

#79697

Purpose

This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB2 (EPHB2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
Rabbit Cav 2.2 W391A pMT2

Plasmid

#58734

Purpose

expression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

Cav2.2 (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

SV40

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-321/+120 Ebox mut1

Plasmid

#35544

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Mutation

T to G at position -96 in promoter

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-321/+120 Ebox mut2

Plasmid

#35545

Depositor

Greg Goodall

Article

Bracken et al Cancer Res. 2008 Oct 1;68(19):7846-54.

Insert

promoter region of miR200b-200a-429 genomic cluster (MIR200B, MIR429, MIR200A Human)

Use

Luciferase

Tags

luciferase

Mutation

T to G at position -52 in promoter

Promoter

none

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter 402 mutation

Plasmid

#89825

Purpose

Fascin promoter for luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Mutation

-370 Smad binding element mutated from CAGAC to T…

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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MK11_HUMAN_D0

Plasmid

#79692

Purpose

This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK11 (MAPK11 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1_NLS_ K682R_K686R_K694R_K709R (4KR) POLH_deltaGG NEDD8

Plasmid

#250151

Purpose

Expresses GFP-tagged K682R_K686R_K694R_K709R POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP and ΔGG NEDD8

Expression

Mammalian

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_NLS_ K682R_K686R_K69R_K709R POLH (4KR)

Plasmid

#246408

Purpose

Expresses K682R_K686R_K694R_K709R POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

K682R, K686R, K694R, K709R

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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