Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,412 results for: APP

Clear keywords
Showing: 9021 - 9040 of 9412 results

  2. pcDNA3.1/Zeo(+)_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182682
    Purpose
    Contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Used as a donor for endogenous NFL tagging via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182682-plasmid-map-sequence-id-363082

    Enlarge

  3. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

    Enlarge

  4. pcDNA3.1/Zeo(+)_mCherry_TKIT NFL intron3/exon4 linker(WT)-3xFLAG donor

    Plasmid
    #182685
    Purpose
    Encodes mCherry and contains a donor sequence with a part of Nefl intron 3, whole Nefl exon 4 and linker-3xFLAG. Can be used as a donor plasmid for the tagging of endogenous NFL via TKIT approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    mouse Nefl intron3-exon4-linker(WT)-3xFLAG donor sequence (Nefl Mouse)
    mCherry
    Expression
    Mammalian
    Promoter
    CMV and NA
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182685-plasmid-map-sequence-id-363019

    Enlarge

  5. ST-Tstem-GFP

    Plasmid
    #162501
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The Tac stem region is inserted within ST6Gal1
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162501-plasmid-map-sequence-id-323506

    Enlarge

  9. JWW-1 human chimeric antibody

    Plasmid
    #66748
    Purpose
    Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.
    Depositor
    Richard Roden
    Article
    Wang et al Clin Vaccine Immunol. 2015 May 13. pii:
    Inserts
    JWW-1 Heavy Chain Gamma
    JWW-1 Light Chain Kappa
    Expression
    Mammalian
    Mutation
    First 3 amino acids of rat light chain constant r…
    Promoter
    mEF1 and rEF1
    Available Since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    66748-plasmid-map-sequence-id-118582

    Enlarge

  10. OPRK1-DuET

    Plasmid
    #213359
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRK1 (OPRK1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213359-plasmid-map-sequence-id-430881

    Enlarge

  16. pCBS-5738

    Plasmid
    #249324
    Purpose
    To express the EPEC DarT2(DLAA)-nScCas9(D10A) fusion protein in experiments for Append Editing
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(DLAA)-nScCas9(D10A)
    Expression
    Yeast
    Mutation
    DarT2(G49D, M86L, R92A, R193A) and nScCas9(D10A)
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249324-plasmid-map-sequence-id-496429

    Enlarge

Showing: 9021 - 9040 of 9412 results